21 July 2015 Optical coherence tomography for cross-sectional imaging of neural activity
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Neurophotonics, 2(3), 035001 (2015). doi:10.1117/1.NPh.2.3.035001
We report a functional optical coherence tomography cross-sectional scanner to detect neural activity using unmyelinated nerves dissected from squid. The nerves, unstained or stained with a voltage-sensitive dye, were imaged in a nerve chamber. Transient phase changes from backscattered light were detected during action potential propagation. The results show that the scanner can provide high spatiotemporal resolution cross-sectional images of neural activity (15  μs/A-line; 0.25  ms/B-scan; ∼8.5×5.5  μm2 in xz). The advantage of this method compared to monitoring a single depth profile z is a dramatic increase in the number of available sites that can be measured in two spatial dimensions xz with lateral scanning; therefore, the study demonstrates that two-dimensional monitoring of small-scale functional activity would also be feasible.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Yi-Jou Yeh, Adam J. Black, David Landowne, Taner Akkin, "Optical coherence tomography for cross-sectional imaging of neural activity," Neurophotonics 2(3), 035001 (21 July 2015). https://doi.org/10.1117/1.NPh.2.3.035001

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