12 October 2015 Differential pathlength factor informs evoked stimulus response in a mouse model of Alzheimer’s disease
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Abstract
Baseline optical properties are typically assumed in calculating the differential pathlength factor (DPF) of mouse brains, a value used in the modified Beer–Lambert law to characterize an evoked stimulus response. We used spatial frequency domain imaging to measure in vivo baseline optical properties in 20-month-old control (n=8) and triple transgenic APP/PS1/tau (3xTg-AD) (n=5) mouse brains. Average μa for control and 3xTg-AD mice was 0.82±0.05 and 0.65±0.05  mm1, respectively, at 460 nm; and 0.71±0.04 and 0.55±0.04  mm1, respectively, at 530 nm. Average μs for control and 3xTg-AD mice was 1.5±0.1 and 1.7±0.1  mm1, respectively, at 460 nm; and 1.3±0.1 and 1.5±0.1  mm1, respectively, at 530 nm. The calculated DPF for control and 3xTg-AD mice was 0.58±0.04 and 0.64±0.04 OD mm, respectively, at 460 nm; and 0.66±0.03 and 0.73±0.05 OD mm, respectively, at 530 nm. In hindpaw stimulation experiments, the hemodynamic increase in brain tissue concentration of oxyhemoglobin was threefold larger and two times longer in the control mice compared to 3xTg-AD mice. Furthermore, the washout of deoxyhemoglobin from increased brain perfusion was seven times larger in controls compared to 3xTg-AD mice (p<0.05).
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Alexander J. Lin, Alexander J. Lin, Adrien Ponticorvo, Adrien Ponticorvo, Anthony J. Durkin, Anthony J. Durkin, Vasan Venugopalan, Vasan Venugopalan, Bernard Choi, Bernard Choi, Bruce J. Tromberg, Bruce J. Tromberg, } "Differential pathlength factor informs evoked stimulus response in a mouse model of Alzheimer’s disease," Neurophotonics 2(4), 045001 (12 October 2015). https://doi.org/10.1117/1.NPh.2.4.045001 . Submission:
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