Acute insertion effects of penetrating cortical microelectrodes imaged with quantitative optical coherence angiography

Daniel X. Hammer; Andrea Lozzi; Adam Boretsky; Cristin G. Welle

doi:10.1117/1.NPh.3.2.025002

19 April 2016 Acute insertion effects of penetrating cortical microelectrodes imaged with quantitative optical coherence angiography

Daniel X. Hammer, Andrea Lozzi, Adam Boretsky, Cristin G. Welle

Author Affiliations +

Neurophotonics, Vol. 3, Issue 2, 025002 (April 2016). https://doi.org/10.1117/1.NPh.3.2.025002

Abstract

The vascular response during cortical microelectrode insertion was measured with amplitude decorrelation-based quantitative optical coherence angiography (OCA). Four different shank-style microelectrode configurations were inserted in murine motor cortex beneath a surgically implanted window in discrete steps while OCA images were collected and processed for angiography and flowmetry. Quantitative measurements included tissue displacement (measured by optical flow), perfused capillary density, and capillary flow velocity. The primary effect of insertion was mechanical perturbation, the effects of which included tissue displacement, arteriolar rupture, and compression of a branch of the anterior cerebral artery causing a global decrease in flow. Other effects observed included local flow drop-out in the region immediately surrounding the microelectrode. The mean basal capillary network velocity for all animals was 0.23 (±0.05 SD) and 0.18 (±0.07 SD) mm/s for capillaries from 100 to 300 μm and 300 to 500 μm, respectively. Upon insertion, the 2-shank electrode arrays caused a decrease in capillary flow density and velocity, while the results from other configurations were not different from controls. The proximity to large vessels appears to play a larger role than the array configuration. These results can guide neurosurgeons and electrode designers to minimize trauma and ischemia during microelectrode insertion.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Daniel X. Hammer, Andrea Lozzi, Adam Boretsky, and Cristin G. Welle "Acute insertion effects of penetrating cortical microelectrodes imaged with quantitative optical coherence angiography," Neurophotonics 3(2), 025002 (19 April 2016). https://doi.org/10.1117/1.NPh.3.2.025002

Published: 19 April 2016

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
17 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY