Filling the gap: adding super-resolution to array tomography for correlated ultrastructural and molecular identification of electrical synapses at the C. elegans connectome

Abstract. Correlating molecular labeling at the ultrastructural level with high confidence remains challenging. Array tomography (AT) allows for a combination of fluorescence and electron microscopy (EM) to visualize subcellular protein localization on serial EM sections. Here, we describe an application for AT that combines near-native tissue preservation via high-pressure freezing and freeze substitution with super-resolution light microscopy and high-resolution scanning electron microscopy (SEM) analysis on the same section. We established protocols that combine SEM with structured illumination microscopy (SIM) and direct stochastic optical reconstruction microscopy (dSTORM). We devised a method for easy, precise, and unbiased correlation of EM images and super-resolution imaging data using endogenous cellular landmarks and freely available image processing software. We demonstrate that these methods allow us to identify and label gap junctions in Caenorhabditis elegans with precision and confidence, and imaging of even smaller structures is feasible. With the emergence of connectomics, these methods will allow us to fill in the gap—acquiring the correlated ultrastructural and molecular identity of electrical synapses.

Filling the gap: adding super-resolution to array tomography for correlated ultrastructural and molecular identification of electrical synapses at the C. elegans connectome 1 Introduction Light microscopy (LM) and electron microscopy (EM) are fundamental imaging methods in the life sciences, each approach having its strengths and limitations. Since the potential gains of combining the two on the same sample are tremendous, many protocols for correlative light and electron microscopy (CLEM) have emerged in recent years. However, CLEM approaches remain challenging due to the vastly different requirements for sample preparation of LM and EM.
Array tomography (AT), first reported by Micheva and Smith, 1 enables three-dimensional (3-D) reconstruction of volumes imaged by scanning electron microscopy (SEM) and LM after immunohistochemistry (IHC). By overlaying aligned SEM and LM images at regions of interest (ROIs) molecular identity, as revealed by IHC, can be put into full context of the ultrastructure. Briefly, ultrathin serial sections of resin-embedded samples are applied to a glass surface, where they are stained with antibodies or other fluorescent dyes. After imaging sections with LM, they are processed and imaged again using SEM. By imaging the same ROI on consecutive serial sections, an image stack with the z-resolution corresponding to the section thickness is generated and used for 3-D reconstruction. Notably, the ultrathin nature of the sections (100 nm and below) elegantly physically breaks the z-resolution diffraction limit of LM, allowing super-resolution in axial direction. 1 The current limitation of AT is lateral LM resolution and correlation. First, it can be very challenging and time consuming to rediscover an ROI during SEM imaging, especially at very high magnification. Second, precise and unbiased correlation requires spatially precise overlaying of structures both seen in EM and LM. Because of these challenges and the limited resolution of conventional LM, AT is in general mostly applied at lower magnifications.
Another possibility for serial CLEM is to utilize the fluorescence of proteins like GFP directly. It is possible to preserve some portion of the fluorescence during freeze substitution and embedding and image it in serial resin sections. 2 In combination with techniques like stimulated emission depletion (STED) microscopy 3 and photoactivated localization microscopy (PALM), 4 fluorescent proteins can even offer super-resolved LM images. 5 Recently, Perkovic et al. 6 reported an elegant method to perform direct stochastic optical reconstruction microscopy (dSTORM) [7][8][9] super-resolution LM on resin sections using in-section fluorescence of uranyl acetate-resistant chemical tags. This resulted in achieving high xand y-resolution of the fluorescent signals. Applied in combination with electron tomography, however, this method still has limited z-resolution of the fluorescence signal and is hard to apply to large numbers of serial sections and thus is more suitable for comparatively smaller volumes. Nanguneri et al. 10 succeeded in applying dSTORM to IHC stained serial resin sections, but did not show direct correlations of dSTORM and EM images of the same sections. Here, we report a method to advance AT for application at very high magnifications using structured illumination microscopy (SIM) 11 in conjunction with correlation to same-section SEM images. We further show that the same principal approach can also be applied using dSTORM to reach even more precise correlation.
The nematode Caenorhabditis elegans is a key model organism in neurobiological research due to its very small, genetically tractable nervous system of exactly 302 neurons in the adult hermaphrodite. It is well suited to establish and rigorously test neuroimaging approaches. 12 This and other advantages of C. elegans, like ease of maintenance and an advanced genetic tool-box, made it the first and still only organism where the reconstruction of its entire neuronal network (connectome) has been undertaken to near completion. 13 Despite its relative simplicity, its annotation remains incomplete, and one of the biggest challenges is the confident mapping of key circuit components, the electrical synapses.
Electrical synapses are formed by connexins 14 and pannexins 15 in vertebrates, and innexins in invertebrates. 16 They form hexamers, so-called connexons/pannexons and innexons, in the plasma membrane, which attach to another hexamer from the membrane of a nearby second cell. 17 This way they form a bridge between the two cells through which ions and metabolites can pass. These intercellular bridges are called gap junctions. Several gap junctions form an electrical synapse and through them electrical and metabolic coupling of the two cells is achieved. 14 C. elegans has at least 25 different innexins, expressed in virtually all cell types and tissues. 18 The large family of innexins may allow for a molecular-level compensation for the small number of cellular components of the C. elegans nervous system. A precise and comprehensive mapping of gap junctions thus provides essential information to understand the connectivity logic of a neuronal network or an entire connectome. Ultrastructural EM methods have mainly characterized gap junctions as defined membrane appositions. 19 However, they are difficult to be unambiguously distinguished by ultrastructural data alone. Emerging CLEM techniques make it now feasible to map gap junctions comprehensively and confidently using molecular labeling.
In a recent publication Collman et al. 20 have shown how improved AT can be efficiently utilized to map synapses in mouse brain subvolumes. However, this analysis focuses on chemical synapses, and conventional fluorescence LM does not have the necessary precision to confidently identify gap junctions. Here, we describe a super-resolution array tomography (srAT) protocol based on high-pressure frozen and freezesubstituted samples that allows us to map gap junctions formed by the innexin UNC-7 in the nervous system of C. elegans. We thus demonstrate an important application of this method and show how mapping of gap junction locations and even discerning their molecular identity in C. elegans and other organisms could be achieved with relative ease, adding a new dimension of connectivity information to connectomes.

Strains
C. elegans were maintained using standard methods. 21 The C. elegans strain used for the gap junction study was ZM1158, which contains an outcrossed integrated array lwIs48[Punc-7:: UNC-7S::GFP], where an innexin UNC-7S fused with GFP was expressed from an endogenous unc-7 promoter. 22,23 UNC-7S appears to be the major form of UNC-7, 22 and for simplicity is referred to as UNC-7 in the main text. For dSTORM analyses, N2 Bristol wild-type was used.

High Pressure Freezing and Freeze Substitution
For high pressure freezing, C. elegans young adults were picked into freezing platelets (recesses 100 and 0 μm, Leica Microsystems) containing 10% bovine serum albumin (BSA) in M9 24 (3.0 g KH 2 PO 4 , 6.0 g Na 2 HPO 4 , 0.5 g NaCl, 1 ml 1 M MgSO 4 NH 4 Cl, H 2 O to 1 l). They were cryoimmobilized with an EM HPM100 (Leica Microsystems) at >20;000 K∕s freezing speed and >2100 bar pressure and stored in liquid nitrogen until freeze substitution. The freeze substitution protocol was adapted from Weimer 25 and Rostaing et al. 26 The samples were processed in an EM AFS2 freeze substitution system (Leica Microsystems). First, they were incubated overnight in 0.1% KMnO 4 in anhydrous acetone at −90°C. The solution was then exchanged with fresh one to efficiently remove residual water. The samples were kept at −90°C for a total of 80 h. Next, the temperature was ramped to −45°C over the course of 11 h. At −45°C the samples were washed four times within 3 h with acetone. Then, acetone was exchanged with one-third ethanol in acetone and incubated for 30 min, then 30 min with two-thirds ethanol in acetone, and finally two times (30 min each) with pure ethanol. Then the temperature was ramped to 4°C over the course of 16 h and the samples were washed again with ethanol two times for 30 min each. Then the samples were removed from the EM AFS2 and incubated in 50% LR White (Medium Grade Acrylic Resin, London Resin Company Ltd.) in ethanol overnight at 4°C. Next, the solution was exchanged with 100% LR White and then again after 1, 4 h, and overnight incubation at 4°C. The specimens were finally embedded in gelatin capsules with either 100% LR White and cured at ∼52°C for at least 48 h or with LR White containing additional accelerator (provided by manufacturer; one drop per 10 ml of resin was used) and cured for at least 48 h at 4°C under UV light and then further 48 h at room temperature under sunlight.

Serial Sections
100-nm serial sections were cut with a histo Jumbo Diamond Knife (Diatome AG, Biel, Switzerland). To ensure ribboning, a thin layer of a 1∶1 mixture of contact adhesive and xylene was added to one side of the block. To make sections easier to find in the light microscope, we also added black pigment to the glue mixture. Microscopic glass slides coated with poly-Llysine (Polysine slides, Thermo Fisher Scientific) or, for dSTORM application, cover slips coated with 0.5% gelatin and 0.05% chromium potassium sulfate 1 were submerged in the knife's boat to receive the sections. The slides/cover slips were then incubated for 20 min at ∼50°C.

Immunostaining and SIM Image Acquisition
Immunostaining was adapted from Micheva and Smith 1 with modifications. Instead of a PAP pen, we used reversibly applicable and removable custom sample chambers made of polydimethylsiloxane (PDMS, Sylgard 184, Dow Corning) for IHC and dSTORM image acquisition. According to the instruction for use, the bulk material is thoroughly mixed 10∶1 with the crosslinker compound, degassed, and subsequently polymerized at 85°C for 4 h in negative molds made from Plexiglas. The resultant rectangular chambers with an inner edge length of 1 cm reversibly adhere to glass slides, creating a barrier for keeping IHC or switching buffers in place (cf. Fig. 1). For initial blocking and rehydration, blocking solution (0.1% BSA and 0.05% Tween 20 in Tris-buffer) was added for 5 min. All incubation steps were performed in humidity chambers at room temperature. The first antibodies were diluted 1∶1000 (monoclonal mouse anti-α-tubulin, 27.8 mg∕ml, Sigma; product number: T5168) or 1∶500 (polyclonal chicken anti-GFP, abcam; product number: ab13970) in blocking solution, centrifuged for 2 min at top speed and applied for 1 h. Sections were washed four times with Tris-buffer (5 min each). Secondary antibodies [for dSTORM application: goat anti-mouse IgG (H+L) Alexa Fluor 647 conjugate, F(ab')2 fragment, Thermo Fisher Scientific; for SIM application: goat anti-chicken IgG (H+L) Alexa Fluor 488 conjugate, Thermo Fisher Scientific, and goat anti-mouse IgG (H+L) Texas Red dye-conjugate, Jackson ImmunoResearch] were also diluted in blocking solution 1∶500 and then applied for 30 min in the dark. After washing Live Hoechst 33342 (Sigma) diluted 1∶10;000 in Tris-buffer was applied, then sections were washed again two times, and finally either PDMS sample chambers were removed and the sections mounted with Mowiol (for SIM application) or sections were kept in Trisbuffer until dSTORM application. All light microscopic analyses were performed within 3 days of immunostaining. SIM image acquisition was performed with an ELYRA S.1 super-resolution structured illumination microscope (Zeiss). Black glue in between sections and the Hoechst staining aided in finding the sections and tissues.

dSTORM Image Acquisition and Data Processing
dSTORM measurements were performed on a custom setup built around a Zeiss Axio Observer.Z1 inverted microscope equipped with a 100× objective with NA ¼ 1.46 (Zeiss). A central region of about 25 × 25 μm 2 of the sample was illuminated by a 150-mW diode laser at 640 nm wavelength (Toptica iBeam smart) and fluorescence was detected with an Andor Ixon Ultra EMCCD camera (DU897U-CSO). The microscope stand is equipped with a 1.6× Optovar lens that directly leads to an effective pixel size of about 100 nm∕px for sufficient sampling of the detected PSFs. 27 Because the setup has two cameras attached to one camera port, the detected light was relayed to the camera by two f ¼ 160 mm lenses (Thorlabs). In this work, the second camera was only used to facilitate finding the specimen by imaging, e.g., Hoechst staining. The shorter wavelengths are directed to the second camera via a longpass filter (Chroma 630 DCXR) placed between the two above-mentioned lenses and imaged with a third lens of the same kind. The longpass filter is never removed from the beam path in order to avoid signal shifts induced by possible slight displacements. For imaging of signals other than Alexa Fluor 647, the sample can additionally be illuminated by 405-, 488-, or 532-nm lasers (Toptica, Laser Quantum). The 640-nm laser was cleaned from spectral noise with a narrow-width bandpass (Semrock MaxDiode LD01-640/8) and focused on the back focal plane of the objective with two lenses (40 and 100 mm, Thorlabs). The light was thereby guided by a dichroic beam splitter (Semrock BrightLine Di01-R405/488/532/635-25x36) and fluorescence was filtered from unwanted light with a rejection filter underneath the beam splitter (Chroma ZET405/488/532/ 642m) and a bandpass in front of the camera (Chroma ET700/75m). A typical measurement consists of a video of the switching molecules with about 15,000 frames acquired at a frame rate of 100 Hz. The measurements were evaluated and visualized with

Contrasting and Carbon Coating
After acquisition of the SIM images, the cover slips were carefully removed, and the whole slide with the attached sections was washed in ddH 2 O to remove the mounting medium. After acquisition of dSTORM images, the cover slips were detached from the PDMS chambers and washed and dried the same way. After drying, the sections were contrasted in 2.5% uranyl acetate in ethanol for 15 min and in 50% Reynolds' lead citrate 29 in decocted ddH 2 O for 10 min. The slides/cover slips were size-reduced using a diamond pen and attached to an SEM pin stub specimen mount. To prevent charging of the specimen, electrically conductive adhesive was added to one side of the glass piece. Finally, the sections were coated with a thin carbon layer to further reduce charging of the sample.

Scanning Electron Microscopy
A field emission scanning electron microscope JSM-7500F (JEOL, Japan) with LABE detector (for back scattered electron imaging at extremely low acceleration voltages) was used with an acceleration voltage of 5 kV, a probe current of 0.3 nA, and a working distance of 8 mm for all SEM images.  From the z-stacks acquired by SIM the z-layer with the highest signal intensity was chosen for correlation and further processed with Fiji 30 (version October 2015), an ImageJ distribution. Background noise was subtracted for each channel individually so that regions with no specific labeling (i.e., pure resin) appeared black, and lookup tables were applied to color the images. They were exported (one image per channel) in portable network graphic (PNG) format and imported into Inkscape, an open source and free vector graphics editor 31 together with the raw SEM images. By reducing the opacity of the SIM images, they can be moved over the SEM images to look for matching structures. The images from the different channels were overlaid, linked, and put into separate layers. Correlation was then performed with the channel for the protein of interest blanked out. To match the structures, SIM images were only rotated and resized, no distortions to the aspect ratio were permitted. After the structures were aligned to satisfaction, the channel with the protein of interest was displayed again. No further manipulations of image location were performed after this stage. All correlated images were cropped to a rectangle so that their sizes and shapes matched exactly. Next, they were saved as individual PNG files and imported into GIMP, an open source and free image editor. 32 The black background was removed from the fluorescence images and all desired channels superimposed. The finished correlated images were exported as PNG files. Figure 2 gives a schematic summary of the correlation process and strategy. Accuracy of correlation was estimated using Fiji. We set the straight line tool to a line width of 10 pixels and used it to determine the peak of the fluorescence signal as well as the peak of highest electron density for each gap junction analyzed in this study. All measurements were performed orthogonally to the gap junction. The difference of the two peaks in nanometers was defined as the divergence of the correlation of this particular gap junction. All correlations were performed three times by three different persons, and thus correlation accuracy of each gap junction was performed three times as well. We pooled all divergences together and calculated the mean and standard deviation to provide a measure of overall accuracy of our correlation method.
Segmentation and 3-D modeling were performed using 3dmod from the free and open source software package IMOD. 33

Identification of Cells
Cell IDs were made based on neurite tracing throughout ∼30 serial EM sections imported into CATMAID, 34 which was compared mainly against the JSH and N2U series published by White et al., 13 covering the same region. We first identified highly characteristic and conserved neurite structures for AIY, AIZ, RIB, RIA, RIG, and SMB in all series, and then, by following stereotypical chemical synapse and gap junction connectivity patterns and other known trajectories based on previous description 13 and those from several fully or partially reconstructed L1 animal synapse patterns from different sectioning angles (M. Zhen and A. Samuel labs, unpublished data), we determined the identity of additional neurites unambiguously.

Super-Resolution Array Tomography Facilitates Mapping of C. elegans Gap Junctions
Our protocol for high pressure freezing, freeze substitution, and LR White embedding at 4°C resulted in a good ultrastructural preservation of the nervous system and other tissues of C. elegans while retaining good antigenicity for several different proteins. To visualize a defined subset of neuronal gap junctions with srAT, we chose a transgenic line that expresses a functional UNC-7::GFP fusion from the native unc-7 promoter. 22,23 The UNC-7 innexin 16 is expressed in many neurons and some body wall muscles. 22 We used an antibody against GFP to stain for UNC-7::GFP, and co-stained for microtubules using an antibody against α-tubulin on ultrathin sections from the young adult C. elegans hermaphrodite. In addition, the sections were stained for heterochromatin with the Hoechst dye. We imaged by SIM several arrays from different regions of the nervous system in different individuals. Here we exemplarily show a series of 29 sections through the retrovesicular ganglion (RVG), a portion of the ventral nerve cord anterior of the excretory pore (cf. Fig. 3). After image acquisition, we prepared the arrays for SEM and electron micrographs of the same regions were obtained (for an overview of the whole workflow see Fig. 1). After correlation of the LM and EM images (Fig. 2), UNC-7::GFP-positive gap junctions were identified within their full ultrastructural context. Figure 3 shows an example section from the series, where a SIM image was correlated with an SEM image. In Fig. 4, we show a serial analysis of 10 consecutive srAT sections, which reveals three UNC-7::GFP positive gap junctions. Only GFP signals that appeared on at least two consecutive sections at the same relative location were considered bona fide labeling. Signals that appeared on single sections were disregarded as potential background. Our analysis revealed high-confidence UNC-7-positive gap junctions (Fig. 5). Several identified gap junctions include those between AVK and SMBD, between AVK and RIG, between RIH and FLP, between AVK and ADE, and between RIM and RIS. These neurons have been previously identified to express UNC-7, 22,23 and on the basis of ultrastructure these gap junctions have been reported in the original C. elegans wiring diagram. 13 In addition, an unambiguous UNC-7::GFP labeled gap junction between ADA and ADF neurons was identified, which, to our knowledge, was not previously reported to express UNC-7.
We also observed UNC-7::GFP intracellular structures that resemble endoplasmic reticulum (ER). ER-localized UNC-7:: GFP signal is likely due to the high expression level of UNC-7::GFP from the multicopy integrated array. If fluorescence microscopy alone is used to assess expression patterns of these lines, results could be very challenging to interpret. However, using srAT we were able to clearly distinguish the ER retention or other expression artifacts from true gap junction signals. srAT is thus suitable for precise determination of protein localization at a subcellular level (for further discussion see below), and it would be feasible to map gap junctions of C. elegans neuronal networks comprehensively.
For a better spatial understanding of the volume of our dataset and the precise positions of the mentioned gap junctions, we segmented identified UNC-7::GFP-positive gap junctions in their cellular context, and generated 3-D models (Fig. 6).

In Combination with dSTORM, srAT Can Be Used for Even More Precise Correlations
We initially applied tubulin staining to aid with correlation, but realized that the resolution of SIM, although much improved compared to conventional fluorescence microscopy 35 and sufficient for our gap junction analysis (see Fig. 7), was insufficient to confidentially discern individual microtubules. In principle, microtubules are excellent internal landmarks for LM/EM correlations for applications that require even higher subcellular precision, such as localizing synaptic vesicle proteins, because they are of uniform size and shape, and are often clearly discernible in electron micrographs. To establish microtubules as such landmarks, we proceeded with dSTORM measurement on sections IHC stained against α-tubulin. Here, we used samples cured in LR White at ∼52°C instead of 4°C. This leads to more extraction of the tissue and synaptic vesicles are not or only barely visible anymore. However, because of that, microtubules become easier to distinguish. Depending on the type of question and tissue, one or the other protocol might be better suited. Figure 8 shows that it was possible to match individual microtubules to distinct dSTORM signals. Thus, we can show that our srAT approach can in principle be applied at even higher magnifications as well. By using the microtubules as intrinsic correlation landmarks, it should be possible to apply our approach for unbiased fitting of proteins with up to now unreached precision and accuracy.

Low-Temperature LR White Embedding Combines Good Structural Preservation with High Antigenicity
AT requires a careful compromise between structure preservation and retention of antigenicity. This is even more important in srAT, where good preservation is needed to analyze smaller structures, and at the same time reliable labeling of proteins is required, in particular when they are of low abundance and locally distributed, like innexins. In their original AT publication Micheva and Smith 1 used the resin LR White cured at ∼52°C. In our hands, this protocol led to heavy extraction of nervous tissue from our C. elegans samples and was not suitable for an analysis of gap junctions, although we took advantage of this characteristic for imaging microtubules with dSTORM (see above). However, we found that curing of LR White at 4°C under UV light resulted in better ultrastructural preservation (cf. Figs. 5 and 8) and comparable antigenicity. In a recent AT publication, Collman et al. 20 compared thermally cured LR White with lowtemperature cured Lowicryl HM20 and found an improved structural preservation for the latter condition. This is consistent with our observation that the curing temperature is of key importance for the preservation of gently fixed tissues for AT.

Correlation Using Intrinsic Landmarks Is Easily Applicable, Precise, and Unbiased
An obstacle of AT is the image correlation. Structures imaged in LM have to be located and reimaged in EM, and then both images need to be superimposed with high precision. To address these problems a number of approaches have been implemented. These methods often rely on external fiducial markers, such as quantum dots 2,36 or finder grids. Since external fiducials are usually applied onto resin sections, it is conceivable that their positions could slightly change in between imaging steps or that some of them get detached. Also, they are difficult to apply, and in the case of quantum dots, offer low contrast in EM.
In our study, we decided to take a more straightforward approach that is easy and comparatively cheap to execute and avoids potential bias. Since multichannel imaging is readily applicable in SIM, we co-imaged suitable structures in different channels from our protein of interest. In this case, staining of heterochromatin was useful for correlations in the nerve ring region that is surrounded by neuronal nuclei. We could easily fit an initial alignment of SEM overview images, since nuclei are clearly visible in electron micrographs. More precise correlation was then made possible by fitting the super-resolved heterochromatin patterns visible in both images (see Fig. 3). The channel-aligned fluorescence signal of the protein of interest is then superimposed without any further positional manipulation, thus avoiding potential bias. With this method, we measured an overall correlation accuracy of 44.8 AE 38.1 nm (mean divergence of fluorescence signal from gap junction electron density ± standard deviation). This approach can be further strengthened using several independent landmarks (depending on the sample of interest). Figure 2 depicts a general diagram of our correlation strategy. We co-stained for microtubules. This was useful for identifying the rough position of the microtubule-rich neuropil tissue, but not useful for precise correlation, because SIM can reach a lateral resolution of about 120 nm and thus cannot reliably resolve individual 25 nm filaments. 35 This changes with the application of dSTORM, however. With this technique, a lateral resolution of 20 nm is typical. 35,37 We were able to identify distinct microtubules in the ventral nerve cord of C. elegans in electron micrographs as well as in dSTORM images (Fig. 8). This equips us with a powerful tool for theoretically even more precise correlation and localization of synaptic proteins of interest, since the microtubules can serve as intrinsic fiducial markers. This level of precision was not necessary in our gap junction analysis, however, and thus we concentrated on SIM in this study. This strategy of choosing suitable endogenous markers and co-imaging them with the protein of interest can be applied to many tissues and questions. Depending on the type of tissue, mitochondria, actin bundles, membranes, or any other structure that is close to the ROI and unambiguously discernible in electron micrographs can potentially be used for precise, unbiased correlation.

Reanalysis of the Whole C. elegans
Connectome Using srAT Is Feasible in the Near Future The dataset shown in Fig. 6 consists of 29 serial worm sections (2.9 μm) in total. From sectioning to correlation, the process took about 5 to 6 workdays for one person. A young adult hermaphrodite C. elegans is just over 1 mm in length, so this would correspond to 10,000 to 12,000 cross sections. Assuming a fulltime dedication, we estimate that it would take one skilled person about 3 years to complete data acquisition of the whole connectome of one worm using our protocols and equipment. Thus, this is theoretically feasible already. However, some steps of the workflow can potentially be improved to speed up the process considerably. Sectioning could be automated by using an automated tape-collecting ultramicrotome (ATUM). 38,39 The resulting tape can be cut into convenient chunks and imaged with a SIM featuring automatic section recognition that can help with imaging, and a multibeam SEM setup as well as an automated registration system could speed up SEM imaging considerably.
In combination with a multiperson concerted effort the overall time estimation for a complete mapping of gap junctions to the C. elegans connectome in a yet to be built pan-innexin GFP line could be brought down to several months.

Conclusion
Recently, block-face techniques like focused-ion beam SEM 40 and serial-block face SEM 41 are used in connectomics research. 42,43 However, AT offers some key advantages compared to these techniques that make it a valuable and complementary alternative. First, AT sections are not lost after imaging, and they can be reimaged for further ultrastructural analysis and reevaluated many times after initial image acquisition. Second, AT adds a whole new level of information by including multichannel IHC and other fluorescence-labeling strategies. Thus, not only ultrastructure alone but also the molecular identities of synapses can be included. 20 In this study, we show that AT paired with super-resolution techniques (srAT) makes it possible to map even gap junctions with a high level of confidence. The major drawback of AT is the comparatively low throughput of imaging. However, in combination with an ATUM, a multibeam SEM, and future developments in automatic staining, image registration, and correlation of the section-tape, this drawback could be overcome. It would become feasible to image very large volumes and to acquire, e.g., the entire C. elegans connectome, including synapses, chemical and electrical, together with their molecular identities. This would be an important step toward a functionally understood, truly complete connectome.