9 February 2017 Imaging extracellular potassium dynamics in brain tissue using a potassium-sensitive nanosensor
Author Affiliations +
Neurophotonics, 4(1), 015002 (2017). doi:10.1117/1.NPh.4.1.015002
Abstract
Neuronal activity results in the release of K + into the extracellular space (ECS). Classically, measurements of extracellular K + ( [ K + ] o ) are carried out using K + -sensitive microelectrodes, which provide a single point measurement with undefined spatial resolution. An imaging approach would enable the spatiotemporal mapping of [ K + ] o . Here, we report on the design and characterization of a fluorescence imaging-based K + -sensitive nanosensor for the ECS based on dendrimer nanotechnology. Spectral characterization, sensitivity, and selectivity of the nanosensor were assessed by spectrofluorimetry, as well as in both wide-field and two-photon microscopy settings, demonstrating the nanosensor efficacy over the physiologically relevant ion concentration range. Spatial and temporal kinetics of the nanosensor responses were assessed using a localized iontophoretic K + application on a two-photon imaging setup. Using acute mouse brain slices, we demonstrate that the nanosensor is retained in the ECS for extended periods of time. In addition, we present a ratiometric ve
Joel Wellbourne-Wood, Theresa S. Rimmele, Jean-Yves Chatton, "Imaging extracellular potassium dynamics in brain tissue using a potassium-sensitive nanosensor," Neurophotonics 4(1), 015002 (9 February 2017). http://dx.doi.org/10.1117/1.NPh.4.1.015002
Submission: Received 9 December 2016; Accepted 19 January 2017
JOURNAL ARTICLE
9 PAGES


SHARE
KEYWORDS
Potassium

Nanosensors

Luminescence

Brain

Dendrimers

Tissues

Sol-gels

Back to Top