14 July 2017 Targeted ischemic stroke induction and mesoscopic imaging assessment of blood flow and ischemic depolarization in awake mice
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Abstract
Despite advances in experimental stroke models, confounding factors such as anesthetics used during stroke induction remain. Furthermore, imaging of blood flow during stroke is not routinely done. We take advantage of in vivo bihemispheric transcranial windows for longitudinal mesoscopic imaging of cortical function to establish a protocol for focal ischemic stroke induction in target brain regions using photothrombosis in awake head-fixed mice. Our protocol does not require any surgical steps at the time of stroke induction or anesthetics during either head fixation or photoactivation. In addition, we performed laser speckle contrast imaging and wide-field calcium imaging to reveal the effect of cortical spreading ischemic depolarization after stroke in both anesthetized and awake animals over a spatial scale encompassing both hemispheres. With our combined approach, we observed ischemic depolarizing waves (3 to 5  mm/min) propagating across the cortex 1 to 5 min after stroke induction in genetically encoded calcium indicator mice. Measures of blood flow by laser speckle were correlated with neurological impairment and lesion volume, suggesting a metric for reducing experimental variability. The ability to follow brain dynamics immediately after stroke as well as during recovery may provide a valuable guide to develop activity-dependent therapeutic interventions to be performed shortly after stroke induction.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Matilde Balbi, Matilde Balbi, Matthieu P. Vanni, Matthieu P. Vanni, Gergely Silasi, Gergely Silasi, Yuki Sekino, Yuki Sekino, Luis Bolanos, Luis Bolanos, Jeffrey M. LeDue, Jeffrey M. LeDue, Timothy H. Murphy, Timothy H. Murphy, } "Targeted ischemic stroke induction and mesoscopic imaging assessment of blood flow and ischemic depolarization in awake mice," Neurophotonics 4(3), 035001 (14 July 2017). https://doi.org/10.1117/1.NPh.4.3.035001 . Submission: Received: 27 February 2017; Accepted: 16 June 2017
Received: 27 February 2017; Accepted: 16 June 2017; Published: 14 July 2017
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