12 July 2017 Utah optrode array customization using stereotactic brain atlases and 3-D CAD modeling for optogenetic neocortical interrogation in small rodents and nonhuman primates
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Abstract
As the optogenetic field expands, the need for precise targeting of neocortical circuits only grows more crucial. This work demonstrates a technique for using Solidworks® computer-aided design (CAD) and readily available stereotactic brain atlases to create a three-dimensional (3-D) model of the dorsal region of area visual cortex 4 (V4D) of the macaque monkey (Macaca fascicularis) visual cortex. The 3-D CAD model of the brain was used to customize an 8×6 Utah optrode array (UOA) after it was determined that a high-density (13×13) UOA caused extensive damage to marmoset (Callithrix jacchus) primary visual cortex as assessed by electrophysiological recording of spiking activity through a 1.5-mm-diameter through glass via. The 8×6 UOA was customized for optrode length (400  μm), optrode width (≤100  μm), optrode pitch (400  μm), backplane thickness (500  μm), and overall form factor (3.45  mm×2.65  mm). Two 8×6 UOAs were inserted into layer VI of macaque V4D cortices with minimal damage as assessed in fixed tissue cytochrome oxidase staining in nonrecoverable surgeries. Additionally, two 8×6 arrays were implanted in mice (Mus musculus) motor cortices, providing early evidence for long-term tolerability (over 6 months), and for the ability to integrate the UOA with a Holobundle light delivery system toward patterned optogenetic stimulation of cortical networks.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Ronald W. Boutte, Ronald W. Boutte, Sam Merlin, Sam Merlin, Guy Yona, Guy Yona, Brandon Griffiths, Brandon Griffiths, Alessandra Angelucci, Alessandra Angelucci, Itamar Kahn, Itamar Kahn, Shy Shoham, Shy Shoham, Steve Blair, Steve Blair, } "Utah optrode array customization using stereotactic brain atlases and 3-D CAD modeling for optogenetic neocortical interrogation in small rodents and nonhuman primates," Neurophotonics 4(4), 041502 (12 July 2017). https://doi.org/10.1117/1.NPh.4.4.041502 . Submission: Received: 31 March 2017; Accepted: 8 June 2017
Received: 31 March 2017; Accepted: 8 June 2017; Published: 12 July 2017
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