1 March 2018 Overexpression of channelrhodopsin-2 interferes with the GABAb receptor-mediated depression of GABA release from the somatostatin-containing interneurons of the prefrontal cortex
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Abstract
Region and cell-type restricted expression of light-activated ion channels is the indispensable tool to study properties of synapses in specific circuits and to monitor synaptic alterations by various stimuli including neuromodulators and behaviors, both ex vivo and in vivo. These analyses require the light-activated proteins or viral vectors for their delivery that do not interfere with the phenomenon under study. Here, we report a case of such interference in which the high-level expression of channelrhodopsin-2 introduced in the somatostatin-positive GABAergic neurons of the dorsomedial prefrontal cortex by an adeno-associated virus vector weakens the presynaptic GABAb receptor-mediated suppression of GABA release.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Lei Liu, Wataru Ito, Alexei Morozov, "Overexpression of channelrhodopsin-2 interferes with the GABAb receptor-mediated depression of GABA release from the somatostatin-containing interneurons of the prefrontal cortex," Neurophotonics 5(2), 025003 (1 March 2018). https://doi.org/10.1117/1.NPh.5.2.025003 . Submission: Received: 18 July 2017; Accepted: 5 February 2018
Received: 18 July 2017; Accepted: 5 February 2018; Published: 1 March 2018
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