The microscopic spatial distribution of biological compounds can be imaged using a coherent anti-Stokes Raman scattering (CARS) microscope. Spatial resolution of less than one micrometer has been achieved, and excellent molecular discrimination using chemically similar molecular species has been obtained using digital image processing techniques.
M. D. Duncan,
T. J. Manuccia,
"Imaging Biological Compounds Using The Coherent Anti-Stokes Raman Scattering Microscope," Optical Engineering 24(2), 242352 (1 April 1985). https://doi.org/10.1117/12.7973483