Principles of pulsed laser double-exposure hologram interferometry are discussed. In this presentation, emphasis is placed on biomedical applications of the method. Procedures for quantitative interpretation of images obtained during reconstruction of holograms are presented and illustrated with representative examples. The procedures for quantitative interpretation of holograms allow determination of translations, rotations, and strains of an object, including determination of and compensation for the object's rigid-body motions. A system for automated interpretation of holograms is also discussed. The results shown relate to in vitro and in vivo studies of tooth and bone motions.