The precision of sine-wave modulation transfer function (MTF) measurements of a color negative film was investigated at three laboratories within the Eastman Kodak Company. A spatial frequency range of 0.19 to 40.0 cycles/mm on Kodacolor Gold 100 film was employed for the measurements. The precision of the measurements at two of the laboratories was found to be equal, with the third set of measurements being less precise. The precision was measured by the standard deviation, at discrete frequencies, of replicate MTF measurements. This work may provide necessary supporting experimentation for an ANSI/ ISO color-negative-film MTF standard, which would enable meaningful comparisons of results between laboratories.