1 August 2005 Computer method for ELISA spot assay analysis
Author Affiliations +
Optical Engineering, 44(8), 083604 (2005). doi:10.1117/1.2009767
The ELISA (enzyme-linked immunosorbent assay) spot assay is a method widely used by immunologists to enumerate cytokine-producing cells within a specific cell population. The ELISA results are presented in an image containing numerous colored spots. We present a method to identify the spots in the image and report on important statistics regarding them. The proposed method employs color analysis in the CIE L*u*v* color space and matched filter technique. The system is trained to obtain a standard color for the spots and calculate the color differences between the spots and background in the L*u*v* space. Matched filters are then used to remove noise and enhance the spots in the color difference map. Intensity thresholding is applied to obtain a binary image in which the pixels in the spots have a grayscale of 1 while the grayscale of the other pixels is 0. A software system is implemented, based on this method, to help immunologists analyze the results obtained from the ELISA.
Chih-Yang Lin, Yu-Tai Ching, Hsuen-Chin Chen, Betty A. Wu-Hsieh, "Computer method for ELISA spot assay analysis," Optical Engineering 44(8), 083604 (1 August 2005). http://dx.doi.org/10.1117/1.2009767

Color difference

Image filtering

Image segmentation

Image enhancement

Optical filters

Binary data

Linear filtering

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