Design and realization of a high-stability optical tweezer

Slawomir Drobczynski; Pascal Hébraud; Jean-Pierre Munch; Sébastian Harlepp

doi:10.1117/1.3257269

1 November 2009 Design and realization of a high-stability optical tweezer

Slawomir Drobczynski, Pascal Hébraud, Jean-Pierre Munch, Sébastian Harlepp

Author Affiliations +

Optical Engineering, Vol. 48, Issue 11, 113601 (November 2009). https://doi.org/10.1117/1.3257269

Abstract

We present a method in which we stabilize mechanically an optical tweezer setup over a period ranging up to 30 min. A feedback loop is used to correct the mechanical and thermal drifts. The position of a fixed object on the sample surface is measured with a CCD device and its fluctuations analyzed and used to maintain its position fixed with three piezoelectric devices. With this setup, we obtain a spatial stability of 1.5 nm in the radial direction and 5 nm in the axial direction. This method opens the route for real-time measurements of kinetics of macromolecules association, at a single molecule level, on very long time scales.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Slawomir Drobczynski, Pascal Hébraud, Jean-Pierre Munch, and Sébastian Harlepp "Design and realization of a high-stability optical tweezer," Optical Engineering 48(11), 113601 (1 November 2009). https://doi.org/10.1117/1.3257269

Published: 1 November 2009

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available