We report a multispectral imaging microscope with dynamic spectral reconfiguration capability. It operates based on a spatial filtering principle. The objective lens images the test specimen onto a dispersive grating surface. A dynamic spatial filter located at the Fourier plane of the 4-f lens group is used to select the desired spectral channel and spectral width for each spectral image diffracted by the grating. The reconfiguration capability enables the optimal spectral band selection for a specific application without hardware component modification. Successful experimental results validate the microscopic multispectral imaging configuration for practical applications.