Time-gated stimulated emission depletion nanoscopy

Yifan Wang; Cuifang Kuang; Zhaotai Gu; Yingke Xu; Shuai Li; Xiang Hao; Xu Liu

doi:10.1117/1.OE.52.9.093107

25 September 2013 Time-gated stimulated emission depletion nanoscopy

Yifan Wang, Cuifang Kuang, Zhaotai Gu, Yingke Xu, Shuai Li, Xiang Hao, Xu Liu

Author Affiliations +

Optical Engineering, Vol. 52, Issue 9, 093107 (September 2013). https://doi.org/10.1117/1.OE.52.9.093107

Abstract

We have designed and built a time-gated continuous wave stimulated emission depletion (CW-STED) nanoscopy to visualize microstructures beyond the diffraction limit. An off-line time-gating detection was performed with the help of time-correlated single-photon counting technique. Experimental results showed that before time-gating, the resolution of our system was about 75 nm with a depletion beam (592 nm) power of 200 mW. By using the off-line time-gating detection, the resolution was further improved to 38 nm. Biological samples were also used to test the performance of our time-gated CW-STED, and a resolution of 70 nm was achieved with a depletion beam (592 nm) power of 85 mW. Detailed principles of time-gated CW-STED were discussed in the text. The time-gated STED provides a better resolution with finite laser power.

Citation Download Citation

Yifan Wang, Cuifang Kuang, Zhaotai Gu, Yingke Xu, Shuai Li, Xiang Hao, and Xu Liu "Time-gated stimulated emission depletion nanoscopy," Optical Engineering 52(9), 093107 (25 September 2013). https://doi.org/10.1117/1.OE.52.9.093107

Published: 25 September 2013

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