3 June 2013 Development of silicon-on-insulator-based nanoporous silicon photonic crystals for label-free DNA detection
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We fabricated a one-dimensional nanoporous silicon photonic crystal on a silicon insulator substrate by a cost-effective electrochemical method as an optical biosensor for the detection of DNA hybridization. In the first step, a transfer matrix method was used to calculate the corresponding reflectivity spectrum for the design of nanoporous silicon photonic crystals. Then silicon-on-insulator-based photonic crystals were prepared by a novel simple electrochemical etching. Genes were hybridized inside the porous silicon (PS) pores by aminopropyltriethoxysilane and glutaraldehyde and detected through frequency resolved reflectance measurements. A detection sensitivity of 17.445  nm/μM is demonstrated with good specific detection. The linear response range covers a concentration range of antifreeze protein gene from 0.625 to 10.000 μM. This high responsivity indicates that the silicon-on-insulator-based PS photonic crystal has significant potential for application in biological micro-electro-mechanical-systems technologies.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Xiaoyi Lv, Xiaoyi Lv, Furu Zhong, Furu Zhong, Zhenhong Jia, Zhenhong Jia, Liangliang Chen, Liangliang Chen, Ji Ma, Ji Ma, Hongyan Zhang, Hongyan Zhang, Zhuangqi Cao, Zhuangqi Cao, Jun Zhou, Jun Zhou, } "Development of silicon-on-insulator-based nanoporous silicon photonic crystals for label-free DNA detection," Optical Engineering 52(6), 064401 (3 June 2013). https://doi.org/10.1117/1.OE.52.6.064401 . Submission:

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