25 September 2013 Time-gated stimulated emission depletion nanoscopy
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Abstract
We have designed and built a time-gated continuous wave stimulated emission depletion (CW-STED) nanoscopy to visualize microstructures beyond the diffraction limit. An off-line time-gating detection was performed with the help of time-correlated single-photon counting technique. Experimental results showed that before time-gating, the resolution of our system was about 75 nm with a depletion beam (592 nm) power of 200 mW. By using the off-line time-gating detection, the resolution was further improved to 38 nm. Biological samples were also used to test the performance of our time-gated CW-STED, and a resolution of 70 nm was achieved with a depletion beam (592 nm) power of 85 mW. Detailed principles of time-gated CW-STED were discussed in the text. The time-gated STED provides a better resolution with finite laser power.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Yifan Wang, Cuifang Kuang, Zhaotai Gu, Yingke Xu, Shuai Li, Xiang Hao, Xu Liu, "Time-gated stimulated emission depletion nanoscopy," Optical Engineering 52(9), 093107 (25 September 2013). https://doi.org/10.1117/1.OE.52.9.093107 . Submission:
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