25 September 2013 Time-gated stimulated emission depletion nanoscopy
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Abstract
We have designed and built a time-gated continuous wave stimulated emission depletion (CW-STED) nanoscopy to visualize microstructures beyond the diffraction limit. An off-line time-gating detection was performed with the help of time-correlated single-photon counting technique. Experimental results showed that before time-gating, the resolution of our system was about 75 nm with a depletion beam (592 nm) power of 200 mW. By using the off-line time-gating detection, the resolution was further improved to 38 nm. Biological samples were also used to test the performance of our time-gated CW-STED, and a resolution of 70 nm was achieved with a depletion beam (592 nm) power of 85 mW. Detailed principles of time-gated CW-STED were discussed in the text. The time-gated STED provides a better resolution with finite laser power.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Yifan Wang, Yifan Wang, Cuifang Kuang, Cuifang Kuang, Zhaotai Gu, Zhaotai Gu, Yingke Xu, Yingke Xu, Shuai Li, Shuai Li, Xiang Hao, Xiang Hao, Xu Liu, Xu Liu, } "Time-gated stimulated emission depletion nanoscopy," Optical Engineering 52(9), 093107 (25 September 2013). https://doi.org/10.1117/1.OE.52.9.093107 . Submission:
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