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17 July 2014 Extended focus imaging in digital holographic microscopy: a review
Marcella Matrecano, Melania Paturzo, Pietro Ferraro
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Optical Engineering, Vol. 53, Issue 11, 112317 (July 2014). https://doi.org/10.1117/1.OE.53.11.112317
Abstract
The microscope is one of the most useful tools for exploring and measuring the microscopic world. However, it has some restrictions in its applications because the microscope’s depth of field (DOF) is not sufficient for obtaining a single image with the necessary magnification in which the whole longitudinal object volume is in focus. Currently, the answer to this issue is the extended focused image. Techniques proposed over the years to overcome the limited DOF constraint of the holographic systems and to obtain a completely in-focus image are discussed. We divide them in two macro categories: the first one involves methods used to reconstruct three-dimensional generic objects (including techniques inherited from traditional microscopy, such as the sectioning and merging approach, or multiplane imaging), while the second area involves methods for objects recorded on a tilted plane with respect to hologram one (including not only the use of reconstruction techniques and rotation matrices, but also the introduction of a numerical cubic phase plate or hologram deformations). The aim is to compare these methods and to show how they work under the same conditions, proposing different applications for each.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Marcella Matrecano, Melania Paturzo, and Pietro Ferraro "Extended focus imaging in digital holographic microscopy: a review," Optical Engineering 53(11), 112317 (17 July 2014). https://doi.org/10.1117/1.OE.53.11.112317
Published: 17 July 2014
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Cited by 38 scholarly publications and 1 patent.
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KEYWORDS
Digital holography
Holograms
3D image reconstruction
Holography
Microscopy
Microscopes
Digital imaging
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