23 December 2016 Tunable narrowband volume holographic imaging spectrometer for macroscopic fluorescence molecular tomography
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Abstract
A simple, compact, and inexpensive tunable volume holographic imaging spectrometer is presented to acquire narrowband multispectral fluorescence measurements. It is constructed with three simple achromatic lenses, a transmission volume hologram, and a detector array. Experiments are carried out to evaluate the performance of this spectrometer. The experimental results indicate that the tunable spectrometer can operate at a wavelength range from 400 to 700 nm. Additionally, this spectrometer can provide a lateral field-of-view greater than 33 mm and a spatial resolution of 1.0 mm under narrow band illumination (full-width-half-maximum bandwidth 20 nm). Multispectral fluorescence molecular tomography experiments are conducted with this spectrometer to test the applicability of narrowband multispectral macroscopy.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Yanlu Lv, Jiulou Zhang, Dong Zhang, Lin Zhang, Jianwen Luo, "Tunable narrowband volume holographic imaging spectrometer for macroscopic fluorescence molecular tomography," Optical Engineering 55(12), 123113 (23 December 2016). https://doi.org/10.1117/1.OE.55.12.123113 . Submission: Received: 20 July 2016; Accepted: 30 November 2016
Received: 20 July 2016; Accepted: 30 November 2016; Published: 23 December 2016
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