23 December 2016 Tunable narrowband volume holographic imaging spectrometer for macroscopic fluorescence molecular tomography
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Abstract
A simple, compact, and inexpensive tunable volume holographic imaging spectrometer is presented to acquire narrowband multispectral fluorescence measurements. It is constructed with three simple achromatic lenses, a transmission volume hologram, and a detector array. Experiments are carried out to evaluate the performance of this spectrometer. The experimental results indicate that the tunable spectrometer can operate at a wavelength range from 400 to 700 nm. Additionally, this spectrometer can provide a lateral field-of-view greater than 33 mm and a spatial resolution of 1.0 mm under narrow band illumination (full-width-half-maximum bandwidth 20 nm). Multispectral fluorescence molecular tomography experiments are conducted with this spectrometer to test the applicability of narrowband multispectral macroscopy.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Yanlu Lv, Yanlu Lv, Jiulou Zhang, Jiulou Zhang, Dong Zhang, Dong Zhang, Lin Zhang, Lin Zhang, Jianwen Luo, Jianwen Luo, } "Tunable narrowband volume holographic imaging spectrometer for macroscopic fluorescence molecular tomography," Optical Engineering 55(12), 123113 (23 December 2016). https://doi.org/10.1117/1.OE.55.12.123113 . Submission: Received: 20 July 2016; Accepted: 30 November 2016
Received: 20 July 2016; Accepted: 30 November 2016; Published: 23 December 2016
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