11 July 2017 Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging of biological cells
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Abstract
Biological cells are usually transparent with a small refractive index gradient. Digital holographic interferometry can be used in the measurement of biological cells. We propose a dual-wavelength common-path digital holographic microscopy for the quantitative phase imaging of biological cells. In the proposed configuration, a parallel glass plate is inserted in the light path to create the lateral shearing, and two lasers with different wavelengths are used as the light source to form the dual-wavelength composite digital hologram. The information of biological cells for different wavelengths is separated and extracted in the Fourier domain of the hologram, and then combined to a shorter wavelength in the measurement process. This method could improve the system’s temporal stability and reduce speckle noises simultaneously. Mouse osteoblastic cells and peony pollens are measured to show the feasibility of this method.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Jianglei Di, Yu Song, Teli Xi, Jiwei Zhang, Ying Li, Chaojie Ma, Kaiqiang Wang, Jianlin Zhao, "Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging of biological cells," Optical Engineering 56(11), 111712 (11 July 2017). https://doi.org/10.1117/1.OE.56.11.111712 . Submission: Received: 15 April 2017; Accepted: 15 June 2017
Received: 15 April 2017; Accepted: 15 June 2017; Published: 11 July 2017
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