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25 May 2017 Multielement surface plasmon resonance immunosensor for monitoring of blood circulation system
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The problems related to the development of a multielement immunosensor device with the prism type of excitation of a surface plasmon resonance in the Kretschmann configuration and with the scanning of the incidence angle of monochromatic light aimed at the reliable determination of the levels of three molecular markers of the system of hemostasis (fibrinogen, soluble fibrin, and D-dimer) are considered. We have analyzed the influence of a technology for the production of a gold coating, modification of its surface, and noise effects on the enhancement of sensitivity and stability of the operation of devices. A means of oriented immobilization of monoclonal antibodies on the surface of gold using a multilayer film of copper aminopentacyanoferrate is developed. For the model proteins of studied markers, the calibrating curves (maximum sensitivity of 0.5  μg/ml) are obtained, and the level of fibrinogen in blood plasma of donors is determined. A four-channel modification of the device with an application of a reference channel for comparing the elimination of the noise of temperature fluctuations has been constructed. This device allows one to execute the express-diagnostics of prethrombotic states and the monitoring of the therapy of diseases of the blood circulation system.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2017/$25.00 © 2017 SPIE
Sergey A. Kostyukevych, Kateryna V. Kostyukevych, Roman V. Khristosenko, Viktor O. Lysiuk, Anastasiya A. Koptyukh, and Nadya L. Moskalenko "Multielement surface plasmon resonance immunosensor for monitoring of blood circulation system," Optical Engineering 56(12), 121907 (25 May 2017).
Received: 2 December 2016; Accepted: 2 May 2017; Published: 25 May 2017

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