Fluorescent sensing with Fresnel microlenses for optofluidic systems

Anna Siudzińska; Andrzej Miszczuk; Jacek Marczak; Katarzyna Komorowska

doi:10.1117/1.OE.56.5.057106

Abstract

The concept of fluorescent sensing in a microchannel equipped with focusing light Fresnel lenses has been demonstrated. The concept employs a line or array of Fresnel lenses generating a line or array of focused light spots within a microfluidic channel, to increase the sensitivity of fluorescent signal detection in the system. We have presented efficient methods of master mold fabrication based on the lithography method and focused ion beam milling. The flexible microchannel was fabricated by an imprint process with new thiolene-epoxy resin with a good ability to replicate even submicron-size features. For final imprinted lenses, the measured background to peak signal level shows more than nine times the increase in brightness at the center of the focal spot for the green part of the spectrum (532 nm). The effectiveness of the microlenses in fluorescent-marked Escherichia coli bacteria was confirmed in a basic fluoroscope experiment, showing the increase of the sensitivity of the detection by the order of magnitude.

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Anna Siudzińska, Anna Siudzińska, Andrzej Miszczuk, Andrzej Miszczuk, Jacek Marczak, Jacek Marczak, Katarzyna Komorowska, Katarzyna Komorowska, } "Fluorescent sensing with Fresnel microlenses for optofluidic systems," Optical Engineering 56(5), 057106 (22 May 2017). https://doi.org/10.1117/1.OE.56.5.057106 . Submission: Received: 15 March 2017; Accepted: 28 April 2017

Received: 15 March 2017; Accepted: 28 April 2017; Published: 22 May 2017

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