28 August 2017 Artificial testing targets with controllable blur for adaptive optics microscopes
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Abstract
This letter proposes a method of configuring a testing target to evaluate the performance of adaptive optics microscopes. In this method, a testing slide with fluorescent beads is used to simultaneously determine the point spread function and the field of view. The point spread function is reproduced to simulate actual biological samples by etching a microstructure on the cover glass. The fabrication process is simplified to facilitate an onsite preparation. The artificial tissue consists of solid materials and silicone oil and is stable for use in repetitive experiments.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Masayuki Hattori, Yosuke Tamada, Takashi Murata, Shin Oya, Mitsuyasu Hasebe, Yutaka Hayano, Yasuhiro Kamei, "Artificial testing targets with controllable blur for adaptive optics microscopes," Optical Engineering 56(8), 080502 (28 August 2017). https://doi.org/10.1117/1.OE.56.8.080502 . Submission: Received: 28 May 2017; Accepted: 3 August 2017
Received: 28 May 2017; Accepted: 3 August 2017; Published: 28 August 2017
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