5 June 2018 Digital field of view correction combined dual-view transport of intensity equation method for real-time quantitative imaging
Author Affiliations +
Abstract
Dual-view transport of intensity equation (TIE) method is an ideal way for quantitative live cell imaging as it has advantages such as real-time imaging, multimode observations, compact setup, and large field of view (FoV). However, due to the image recorder installation error, the inevitable FoV mismatch between the captured under- and over-focus intensities reduces the accuracy in both amplitude and phase retrievals. Here, to eliminate this undesired FoV mismatch, the phase correlation-based digital FoV correction is adopted to recognize and compensate the rotation, scale, and translation between the under- and over-focus images. Both the numerical simulations as well as the experiments in standard sample detection and quantitative live cell imaging prove that the digital FoV correction combined dual-view TIE method can maintain the consistence of the dual FoVs, thus guaranteeing the high-accurate amplitude and phase computations, proving the proposed method is a promising quantitative live cell imaging tool in various applications such as biological observations and medical diagnostics.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Qingtao Gong, Qingtao Gong, Qi Wei, Qi Wei, Jing Xu, Jing Xu, Yan Kong, Yan Kong, Zhilong Jiang, Zhilong Jiang, Weiying Qian, Weiying Qian, Yueyue Zhu, Yueyue Zhu, Liang Xue, Liang Xue, Fei Liu, Fei Liu, Cheng Liu, Cheng Liu, Shouyu Wang, Shouyu Wang, } "Digital field of view correction combined dual-view transport of intensity equation method for real-time quantitative imaging," Optical Engineering 57(6), 063102 (5 June 2018). https://doi.org/10.1117/1.OE.57.6.063102 . Submission: Received: 17 February 2018; Accepted: 18 May 2018
Received: 17 February 2018; Accepted: 18 May 2018; Published: 5 June 2018
JOURNAL ARTICLE
10 PAGES


SHARE
Back to Top