Flavin mononucleotide (FMN) plays an important role in living systems. Accurate probing and characterizing of FMN is the key to understanding the contribution of FMN coenzyme in biological processes. We demonstrate a way to get a bright surface-enhanced Raman spectroscopy (SERS) signal while simultaneously quenching the fluorescence background through silver nanorod (AgNR) array substrates fabricated by the oblique angle deposition method. The AgNR array substrates show a good SERS activity with an enhancement factor of ∼4.2 × 104. Meanwhile, the characteristic peaks of FMN are assigned by the density function theory calculation. An exponential relationship is demonstrated between SERS intensity and FMN concentration in the concentration range of 0.1 to 100 mg / L. This SERS-based method enabled the rapid and highly sensitive detection of FMN, holding great promise for expanding SERS application in biochemistry and food safety fields.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither SPIE nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the SPIE website.