Age effects on brain oxygenation during hypercapnia

Rodolfo Gabriel Gatto; William E. Hoffman; Mark Mueller M.D.; Chanannait Paisansathan M.D.; Fady T. Charbel M.D.

doi:10.1117/1.2804705

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1 November 2007 Age effects on brain oxygenation during hypercapnia

Rodolfo Gabriel Gatto, William E. Hoffman, Mark Mueller M.D., Chanannait Paisansathan M.D., Fady T. Charbel M.D.

Author Affiliations +

J. of Biomedical Optics, 12(6), 062113 (2007). https://doi.org/10.1117/1.2804705

Abstract

Previous studies showed that the cerebrovasodilation response to hypercapnia is attenuated with aging. The purpose of this study was to determine if normal aging attenuates increases in brain oxygenation during hypercapnia. Prefrontal cortex oxyhemoglobin (OHb) and deoxyhemoglobin (HHb) concentrations were measured in 13 healthy subjects ages 26 to 59 years using a frequency domain tissue oximeter. Measurements were obtained under the following conditions: (1) subject awake breathing spontaneously, (2) during mask ventilation with 21% oxygen, (3) mask ventilation with 100% oxygen, (4) 100% oxygen in a rebreathing circuit to increase end-tidal CO₂. Under baseline conditions breathing room air, there was a negative correlation between baseline OHb and age (r=-0.60, P<0.05). Ventilation with 100% oxygen increased OHb without a change in total hemoglobin and no affect of age. During mask rebreathing, end-tidal CO₂ increased from 39.5±5.0 mm Hg (millimeters of mercury) to 56.5±5.7 mm Hg, which produced significant increases in OHb and total blood volume that were negatively correlated with age (r=-0.67, P<0.05) and positively correlated to baseline OHb (r=0.60, P<0.05). These results indicate that OHb concentrations decreased with age, consistent with attenuated cerebral vasodilation during hypercapnia.

1. Introduction

Frequency domain–near-infrared spectroscopy (FD-NIRS) allows the measurement of absolute brain oxyhemoglobin (OHb) and deoxyhemoglobin (HHb) concentrations.¹ Reports indicate that cerebral vasodilatory capacity during hypercapnia is decreased with aging.² Safonova, ³ found that cerebral vasomotion is decreased and there is an attenuated increase in OHb during breath holding in volunteers aged 38 to 56 compared to younger subjects. Other reports indicate that increases in OHb related to mental stimulation are less in an aged population.⁴ This suggests that cerebrovasodilation and enhanced brain oxygenation produced by brain stimulation or hypercapnia are attenuated with aging.

We hypothesized that decreases in baseline OHb and total hemoglobin (tHb) could be related to decreased blood flow and cerebrovasodilatory capacity in the aged.^{5, 6, 7} The purpose of this study was to determine if baseline OHb concentration and OHb reactivity to hypercapnia are affected by aging. These results could be used to identify normal versus pathological cerebrovascular responses associated with aging.

2. Methods

These studies were approved by the institutional review board, and 13 healthy volunteers with no known cerebrovascular disease gave signed consent.

2.1.

Instrumentation

The Oxiplex TS (ISS Inc., Champaign, Illinois) is a FD-NIRS oximeter with multidistance light sources to monitor OHb, HHb, and tHb concentrations.⁸ This device utilized multidistance near-infrared light modulated at an RF frequency of $110 MHz$ to determine regional hemoglobin content in brain tissue in absolute concentration $(μ mol ∕ L)$ .⁹

Eight laser diodes were coupled to the oximeter by optical fibers that emitted light at wavelengths of either 690 or $830 nm$ and one detector. The four source-detector distances of the probe were 2.5, 3.0, 3.5, and $4.0 cm$ in all patients. The probe was placed on the right forehead $2 cm$ above the eyebrow and $1 cm$ lateral from the midline and shielded from outside light. OHb and HHb were determined every $2 s$ . The $t Hb$ was calculated as the sum of OHb and HHb and brain oxygen saturation $({SbO}_{2})$ calculated as OHb divided by tHb and multiplied by 100.

Other data, including noninvasive mean blood pressure, heart rate, inspired oxygen fraction, and end-tidal ${CO}_{2}$ $({ETCO}_{2})$ were collected by computer from a Datex-Ohmeda anesthetic monitor (General Electric, Madison, Wisconsin) every $10 s$ .

2.2.

Protocol

Treatment 1 was made with the subject recumbent in a quiet room, with reduced ambient lighting and breathing room air for $10 \min$ . Treatment 2 was made with the subject breathing via face mask from the circuit of a Narkomed 2B anesthesia machine (North American Dragger, Telford, Pennsylvania) with ${CO}_{2}$ absorbent removed and gas flow of medical air (21% oxygen) at $10 L ∕ \min$ for $10 \min$ . In treatment 3, medical air was discontinued and 100% $O_{2}$ was given at $10 L ∕ \min$ for $10 \min$ . In treatment 4, the gas flow of 100% oxygen was reduced to 200 to $500 cc ∕ \min$ and the adjustable pressure-limiting valve of the circle system was closed, and the subject was allowed to rebreath the gases in the circuit until the volunteer indicated a desire to stop. After rebreathing to an ${ETCO}_{2}$ of $50 mm Hg$ (millimeters of mercury) or greater, the face mask was removed and another $5 \min$ breathing room air was allowed before the monitors were removed.

Data are reported as mean $\pm$ standard deviation. Data in each subject were compared over time using a repeated measures analysis of variance with Tukeys tests used for post hoc evaluation. Separate repeated measures analyses of covariance were used to investigate the impact of age, blood pressure, or heart rate on the change in OHb from baseline to the mask rebreathing treatment. OHb, HHb, tHb, and ${SbO}_{2}$ were correlated with age by Pearson product moment correlation. The group size $(n = 13)$ was calculated to detect a 5% change in total hemoglobin content with a power of 90%, assuming a standard deviation of 5% from normal levels of $40 μ mol ∕ L$ and a significance level of 0.05. The average baseline variation of total hemoglobin under steady state unanesthetized conditions in all patients was 2.0%.

3. Results

The age range of the 13 volunteers in this study was 26 to 59 years, and there were 10 males and 3 females. There were no changes in blood pressure or heart rate until mask rebreathing to increase ${ETCO}_{2}$ , and this produced a significant increase in blood pressure and heart rate (Table 1 ). Under baseline conditions, breathing room air, volunteer age was negatively correlated to baseline OHb concentration ( $r = - 0.60$ , $P < 0.05$ , Fig. 1 ).

Fig. 1

Baseline brain oxyhemoglobin concentration plotted as a function of age. There was a negative correlation between OHb and age $(r = - 0.61)$ as indicated in the figure.

Analysis of covariance indicated there was a significant increase in OHb from baseline to mask rebreathing (OHb: $F = 14.48$ , $P < 0.05$ ) and this was significantly influenced by age ( $OHb \times age$ : $F = 3.97$ , $P < 0.05$ ). The increase in OHb per $mm Hg$ change in ${CO}_{2}$ was negatively correlated with age ( $r = - 0.67$ , $P < 0.05$ , Fig. 2 ) and positively correlated to baseline OHb ( $r = 0.60$ , $P < 0.05$ ). There was no significant effect of the increase in heart rate or blood pressure during hypercapnia and the increase in OHb as determined by analysis of covariance.

Fig. 2

OHb reactivity during hypercapnia, plotted as a function of age. There was a negative correlation $(r = - 0.67)$ as indicated in the figure.

The tHb increased 9% during hypercapnia compared to baseline and this was negatively correlated with age (Fig. 3 ).

Fig. 3

(a) Total hemoglobin percent changes during oxygen and ${CO}_{2}$ challenges expressed as $mean \pm standard$ deviation, $* = p < 0.05$ compared to previous measure. (b) tHb ${CO}_{2}$ reactivity plotted as a function of age.

Table 1

Brain tissue oxygenation, ETCO2 , blood pressure, and heart rate in 13 subjects expressed as mean±standard deviation.

	Baseline (room air)	Mask (FiO221%)	Mask (FiO2100%)	Mask (100% O2 rebreathing)
${SbO}_{2}$ (%)	$57 \pm 7$	$57 \pm 7$	$62 \pm 7$ (*)	$66 \pm 8$ (*)
OHb $(μ mol ∕ L)$	$23.8 \pm 7.8$	$23.9 \pm 8.2$	$26.1 \pm 9.0$ (*)	$30.1 \pm 10.6$ (*)
HHb $(μ mol ∕ L)$	$18.0 \pm 7.2$	$17.7 \pm 7.2$	$15.8 \pm 6.5$ (*)	$15.4 \pm 6.2$
tHb $(μ mol ∕ L)$	$41.7 \pm 13.6$	$41.6 \pm 14.1$	$41.8 \pm 14.1$	$45.5 \pm 14.7$ (*)
${ETCO}_{2}$ $(mm Hg)$		$39.9 \pm 4.4$	$39.5 \pm 5.0$	$56.5 \pm 5.7$ (*)
MABP $(mm Hg)$	$109 \pm 13$	$110 \pm 13$	$114 \pm 13$	$124 \pm 13$ (*)
HR $(\min^{- 1})$	$74 + 12$	$76 + 13$	$74 + 11$	$81 + 14$ (*)

^(*) p> 0.05 compared to previous treatment.

FiO2 =fractional inspired oxygen concentration, MABP=mean arterial blood pressure, HR=heart rate.

4. Discussion

These results indicate that OHb concentration decreased as a function of aging in volunteers under baseline conditions. There was no age-related effect on brain oxygenation during inspiration of 100% oxygen but the increase in OHb and tHb during hypercapnia was age dependent. The response to ${CO}_{2}$ was also correlated with baseline OHb concentration. These results are consistent with previous reports that cerebral blood flow and the cerebral vasodilatory response to ${CO}_{2}$ were attenuated with age.^{2, 3, 10} Our data indicate that age-attenuated cerebral vasodilation during hypercapnia is related to decreased baseline OHb concentrations.

There is evidence that cerebral hemodynamic changes occur with aging that inhibit maintenance of brain oxygenation during anesthetic and physiological challenges. During brain stimulation produced by oculomotor activity or mental function tasks, cerebral vasodilation and increases in brain oxygenation are attenuated with aging.^{11, 12} Similarly, cerebral vasodilation produced by hypercapnia is attenuated in aged subjects.² During anesthesia, it is reported that aged patients are at greater risk for brain hypoxia due to an inability to maintain cerebral blood flow during decreases in arterial blood pressure.^{13, 14} Our data suggest that cerebrovascular reactivity and oxygenation during hypercapnia are attenuated with aging.

These results show OHb concentrations decreased with age. It is not clear whether age-related decreases in OHb concentration are linked to decreased cerebral vasodilatory capacity. Buxton⁷ showed that enhanced brain oxygenation during brain stimulation is mediated by cerebral vasodilation and an increase in cerebral blood volume. Huppert ¹⁵ confirmed this relationship and indicated that the ratio was 0.6 for the change in cerebral blood volume relative to the increase in cerebral blood flow. In this study, tHb, an indication of blood volume, increased during rebreathing. This is consistent with cerebral vasodilation. Because OHb decreased with age, this may indicate that decreased brain vascular volume under baseline conditions is related to lower vasodilatory capacity.

It is reported that normal aging can decrease cerebral blood flow in the forebrain and anterior cingulate gyrus.^{5, 6} This suggests that the decrease in OHb that was related to age may be related to a decrease in cerebral blood flow. In addition, attenuated cerebrovascular reactivity to ${CO}_{2}$ is reported in the aged.¹⁰ The decrease in OHb and tHb reactivity to changes in ${CO}_{2}$ with age may be linked to decreased cerebral blood flow.

It is possible that changes in scalp blood flow during hypercapnia affected brain tissue measures. No attempt was made to correct for skin and scalp flow in this study. Noninvasive NIRS is complicated because the light path to the brain tissue is intersected by scalp, skull, and cerebrospinal fluid. Unless the layered structure is accounted for, measurements could be inaccurate.¹⁶ The dominant factor in near-infrared tissue light transport is scattering, 10 times more probable than absorption. The problem is to determine the absorption coefficient of hemoglobin separately from the scattering coefficient of the tissue. Steady state intensity near-infrared measurements ascertain the relative absorption of the tissues with no explicit knowledge of the scattering properties available.⁹ FD-NIRS uses frequency modulated multidistance light arrays to measure scattering and absorption and determine absolute brain concentrations of OHb and HHb in brain tissue separate from scalp and skull.^{1, 8} These studies suggest that contamination from nonbrain tissue is minimal.

We noted in this study that there were two clusters of data points above and below the regression lines for baseline OHb—OHb reactivity and tHb reactivity with respect to age. This suggests that throughout the age range of this study, there is a biphasic grouping of subjects with high and low OHb and tHb reactivity, related to baseline OHb. Our finding agrees with a report that patients with low baseline OHb had an attenuated brain oxygenation response to stressful stimulation.¹⁷ It is not clear why there is a biphasic grouping of subjects with high and low baseline OHb, but this is related to ${CO}_{2}$ reactivity.

It is a problem in this study that the sequence of treatments was the same in all subjects and was not randomized. An error of measurement may have occurred due to the systematic treatment. We did not observe a change in brain oxygenation when the volunteer breathed 21% oxygen spontaneously or by mask. This suggests there was stability in the measurement over time. We included 100% oxygen as one of the treatments in order to maintain adequate oxygen in the circuit and avoid any risk of hypoxia during the rebreathing procedure. The changes in OHb and HHb with 100% oxygen alone were consistent with our expectations that there was no age-related difference in the ability to provide oxygen to the brain. We did see age-related differences in brain oxygenation during hypercapnia. This is probably related to an attenuated cerebrovasodilatory to ${CO}_{2}$ in normal aging.¹⁰

It is possible that increases in blood pressure had an effect to increase OHb independent of the cerebral vasodilating effect of hypercapnia.¹⁸ However, neither heart rate nor blood pressure were significantly related to the increase in OHb during hypercapnia by analysis of covariance. Also, increases in ${ETCO}_{2}$ would not disrupt autoregulation. However, increases in cerebral blood flow during hypercapnia may be enhanced by simultaneous increases in blood pressure. In this study, there was no age-related effect of hypercapnia on blood pressure, indicating that the age-related increase in OHb was not pressure related.

In conclusion, these results confirm that OHb decreased as a function of age and suggest that this is related to attenuated brain oxygenation during hypercapnia. The data suggest that baseline OHb concentrations may predict cerebral vasodilation during physiological challenges. This agrees with recent studies that brain hypoxia may occur in neurosurgical patients under normal anesthetic conditions if awake baseline OHb measures are low.¹³ The clinical importance of future studies may be to identify the influence of brain pathology with respect to normal aging.

Acknowledgments

This study was funded by the departments of anesthesiology and neurosurgery, University of Illinois at Chicago.

References

1.

J. Choi, “Noninvasive determination of the optical properties of adult brain: Near-infrared spectroscopy approach,” J. Biomed. Opt., 9 221 –229 (2004). https://doi.org/10.1117/1.1628242 1083-3668 Google Scholar

2.

H. Ito, I. Kanno, M. Ibaraki, and J. Hatazawa, “Effect of aging on cerebral vascular response to Paco2 changes in humans as measured by positron emission tomography,” J. Cereb. Blood Flow Metab., 22 997 –1003 (2002). https://doi.org/10.1097/00004647-200208000-00011 0271-678X Google Scholar

3.

L. P. Safonova, A. Michalos, U. Wolf, M. Wolf, D. M. Hueber, J. H. Choi, R. Gupta, C. Polzonetti, W. W. Mantulin, and E. Gratton, “Age-correlated changes in cerebral hemodynamics assessed by near-infrared spectroscopy,” Arch. Gerontol. Geriatr., 39 207 –225 (2004). 0167-4943 Google Scholar

4.

M. J. Herrmann, A. Walter, A. C. Ehlis, and A. J. Fallgatter, “Cerebral oxygenation changes in the prefrontal cortex: Effects of age and gender,” Neurobiol. Aging, 27 888 –894 (2006). 0197-4580 Google Scholar

5.

K. Inoue, H. Ito, R. Goto, M. Nakagawa, S. Kinomura, T. Sato, K. Sato, and H. Fukuda, “Apparent CBF decrease with normal aging due to partial volume effects: MR-based partial volume correction on CBF SPECT,” Ann. Nucl. Med., 19 283 –290 (2005). 0914-7187 Google Scholar

6.

K. Takahashi, S. Yamaguchi, S. Kobayashi, and Y. Yamamoto, “Effects of aging on regional cerebral blood flow assessed by using technetium

Tc 99 m

hexamethylpropyleneamine oxime single-photon emission tomography with 3D stereotactic surface projection analysis,” AJNR Am. J. Neuroradiol., 26 2005 –2009 (2005). 0195-6108 Google Scholar

7.

R. B. Buxton, K. Uludag, D. J. Dubowitz, and T. T. Liu, “Modeling the hemodynamic response to brain activation,” Neuroimage, 23 ((Suppl. 1)), S220 –S233 (2004). 1053-8119 Google Scholar

8.

R. Gatto, W. Hoffman, M. Mueller, A. Flores, T. Valyi-Nagy, and F. T. Charbel, “Frequency domain near-infrared spectroscopy technique in the assessment of brain oxygenation: A validation study in live subjects and cadavers,” J. Neurosci. Methods, 157 274 –277 (2006). 0165-0270 Google Scholar

9.

S. Fantini, D. Hueber, M. A. Franceschini, E. Gratton, W. Rosenfeld, P. G. Stubblefield, D. Maulik, and M. R. Stankovic, “Non-invasive optical monitoring of the newborn piglet brain using continuous-wave and frequency-domain spectroscopy,” Phys. Med. Biol., 44 1543 –1563 (1999). https://doi.org/10.1088/0031-9155/44/6/308 0031-9155 Google Scholar

10.

K. Groschel, C. Terborg, S. Schnaudigel, T. Ringer, A. Riecker, O. W. Witte, and A. Kastrup, “Effects of physiological aging and cerebrovascular risk factors on the hemodynamic response to brain activation: A functional transcranial Doppler study,” Eur. J. Neurol., 14 125 –131 (2007). Google Scholar

11.

V. S. Mattay, F. Fera, A. Tessitore, A. R. Hariri, K. F. Berman, S. Das, A. Meyer-Lindenberg, T. E. Goldberg, J. H. Callicott, and D. R. Weinberger, “Neurophysiological correlates of age-related changes in working memory capacity,” Neurosci. Lett., 392 32 –37 (2006). 0304-3940 Google Scholar

12.

M. Raemaekers, M. Vink, M. P. van den Heuvel, R. S. Kahn, and N. F. Ramsey, “Effects of aging on BOLD fMRI during prosaccades and antisaccades,” J. Cogn Neurosci., 18 594 –603 (2006). 0898-929X Google Scholar

13.

W. Hoffman, R. Gatto, V. L. Baughman, C. Paisansathan, and F. T. Charbel, “Brain hypoxic changes during neuroanesthesia,” J. Neurosurg. Anesthesiol., 0898-4921 Google Scholar

14.

A. Casati, G. Fanelli, P. Pietropaoli, R. Proietti, R. Tufano, G. Danelli, G. Fierro, G. De Cosmo, and G. Servillo, “Continuous monitoring of cerebral oxygen saturation in elderly patients undergoing major abdominal surgery minimizes brain exposure to potential hypoxia,” Anesth. Analg. (Baltimore), 101 740 –747 (2005). 0003-2999 Google Scholar

15.

T. J. Huppert, R. D. Hoge, S. G. Diamond, M. A. Franceschini, and D. A. Boas, “A temporal comparison of BOLD, ASL, and NIRS hemodynamic responses to motor stimuli in adult humans,” Neuroimage, 29 368 –382 (2006). 1053-8119 Google Scholar

16.

F. Fabbri, M. E. Henry, P. F. Renshaw, S. Nadgir, B. L. Ehrenberg, M. A. Franceschini, and S. Fantini, “Bilateral near-infrared monitoring of the cerebral concentration and oxygen-saturation of hemoglobin during right unilateral electro-convulsive therapy,” Brain Res., 992 193 –204 (2003). 0006-8993 Google Scholar

17.

C. Paisansathan, W. Hoffman, R. Gatto, V. L. Baughman, M. Mueller, and F. T. Charbel, “Increased brain oxygenation during intubation-related stress,” Eur. J. Anaesthesiol., 0265-0215 Google Scholar

18.

B. P. Wagner and J. Pfenninger, “Dynamic cerebral autoregulatory response to blood pressure rise measured by near-infrared spectroscopy and intracranial pressure,” Crit. Care Med., 30 2014 –2021 (2002). 0090-3493 Google Scholar

Citation Download Citation

Rodolfo Gabriel Gatto, William E. Hoffman, Mark Mueller M.D., Chanannait Paisansathan M.D., and Fady T. Charbel M.D. "Age effects on brain oxygenation during hypercapnia," Journal of Biomedical Optics 12(6), 062113 (1 November 2007). https://doi.org/10.1117/1.2804705

Published: 1 November 2007

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