Comparison of 1540-nm laser-induced injuries in ex vivo and in vitro rabbit corneal models

Nicole McPherson; Thomas E. Eurell; Thomas E. Johnson

doi:10.1117/1.2822371

1 November 2007 Comparison of 1540-nm laser-induced injuries in ex vivo and in vitro rabbit corneal models

Nicole McPherson, Thomas E. Eurell, Thomas E. Johnson

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Journal of Biomedical Optics, Vol. 12, Issue 6, 064033 (November 2007). https://doi.org/10.1117/1.2822371

Abstract

Despite the increasing use of infrared lasers in medical, industrial, and military settings, data on threshold radiant exposures and median effective dose (ED₅₀) as they relate to laser-tissue interaction are limited. Our goals were to determine the ED50 for single-pulse, 1540-nm laser exposures in ex vivo and in vitro rabbit corneal models and to characterize the histopathological changes associated with the laser-tissue interaction. An erbium-glass laser was used to deliver single, 1540-nm wavelength pulses to 27 ex vivo and 24 in vitro rabbit corneal models. The ex vivo model was exposed to single pulses of 0.8-ms duration and radiant energies ranging from 17.61 J/cm² to 42.26 J/cm². The in vitro corneal models were exposed to single pulses of 0.8 ms duration and had radiant exposures ranging from 14.87 to 29.72 J/cm². Tissue exposure sites were observed for presence of a lesion immediately post-exposure and 24 h after exposure. Histopathological evaluations of tissue exposure sites were conducted 24 h after exposure. The ED₅₀ was determined to be 21.24 J/cm² for the in vitro rabbit corneal models and 30.86 J/cm² for the ex vivo corneal models. Both the in vitro and ex vivo models displayed similar histopathological responses of tissue necrosis and epithelial cell proliferation.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Nicole McPherson, Thomas E. Eurell, and Thomas E. Johnson "Comparison of 1540-nm laser-induced injuries in ex vivo and in vitro rabbit corneal models," Journal of Biomedical Optics 12(6), 064033 (1 November 2007). https://doi.org/10.1117/1.2822371

Published: 1 November 2007

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