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1 September 2008 Errata: Quantitative two-photon flow cytometry—in
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Due to a publisher oversight, in this article [J. Biomed. Opt. 13, 034008 (2008)], Fig. 1, 5, 12, 14 were not printed in color. The figures are therefore reproduced here in color. The phrase “(Color online only.)” was removed from the captions for Figs. 5, 12, 14 in the online version of the article.

Fig. 1

Two-beam two-channel two-photon flow cytometry setup (T3TC).

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Fig. 5

Confocal microscopy images of (a) PMBC stained with CFSE (green) and DeepRed (red), (b) green channel of KB cells stained with G5-FI-FA and G5-DR-FA, and (c) Red channel of KB cells stained with G5-FI-FA and G5-DR-FA. The details are given in Sec. 4.

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Fig. 12

(a) S channel (green) and L channel (red) traces from an ear blood vessel of a CD-1 mouse injected with 2.3×109 yellow-green fluorescent (Ex505/Em515) 2.0-μm microspheres at time zero and 11min after. The control traces are shown to the left of the orange dashed line. The orange and blue dashed lines corresponds to the first and second injection, respectively. Each spike in the S channel corresponds to the fluorescent burst from microspheres passing through the excitation region. (b) Blow up of the dual-channel raw data showing detail of individual fluorescent peaks. (c) Number of detected events at different time points before and after injection. Each time point is represented by number of peaks above the background threshold during a period of 107s . (d) S channel (green) and L channel (red) traces from an ear blood vessel of a NU/NU CD-1 mouse injected with 3.6×106 DeepRed-labeled splenocytes at time zero and 25min after. The control traces are shown to the left of the orange dashed line. The orange, blue, black, and magenta dashed lines correspond to the first injection, the second injection, 2h after initial injection, and 1day after, respectively. Each spike in the L channel corresponds to the fluorescent burst from DeepRed-labeled splenocytes passing through the excitation region. (e) Magnified dual-channel raw data. (f) Number of detected events at different time points before and after injection. Each time point is represented by the number of peaks above the background threshold during a period of 214s .

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Fig. 14

(a) S channel (green) and L channel (red) traces from a blood vessel in the ear of a NU/NU CD-1 mouse injected with 300μL (5μM) DeepRed solution at time zero. The control traces are shown to the left of the orange dashed line. The orange and black dashed lines correspond to the initial injection and 1.5h after initial injection, respectively. Each spike in the L channel corresponds to the fluorescent burst from DeepRed fluorophores in cells passing through the excitation region. (b) Magnified dual-channel raw data at the time of injection showing the background rise in the L channel after injection of free DeepRed dye solution. Individual fluorescent peaks above the background noise can also be observed in the L channel after the injection of the free DeepRed dye solution. (c) The background two-photon excited fluorescent signal in the L channel at different time points after the injection of DeepRed dye solution. The fluorescent peaks above threshold were not counted in the background signal. The red dashed line is the linear fit of the log value of two-photon fluorescence against time. (d) Number of detected events at different time points before and after injection. Each time point is represented by the number of peaks above the background threshold during a period of 214seconds . (e) The 20-min peak frequency dynamics in the blood vessel of a NU/NU CD-1 mouse after the injection of 300μL of DeepRed solution. The frequency was calculated as the number of peaks in the L channel with 214 (red) or 107s (blue) time duration. Both axes are on a log scale. The coefficient of the linear fit (black dashed line) is the average of the coefficients from both of the decay curves.

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All online versions of the article were corrected on 9 October 2008.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Frank C. Zhong, Eric R. Tkaczyk, Thommey P. Thomas, Jing Yong Ye, Andrzej Myc, Anna U. Bielinska, Zhengyi Cao, Istvan J. Majoros, Balazs Keszler, James R. Baker Jr., and Theodore B. Norris "Errata: Quantitative two-photon flow cytometry—in," Journal of Biomedical Optics 13(5), 059801 (1 September 2008). https://doi.org/10.1117/1.2993165
Published: 1 September 2008
JOURNAL ERRATUM
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