Study of gold nanoparticles and live cells interactions by using planar evanescent wave excitation

Chia-Wei Lee; En-Hong Lin; Ji-Yen Cheng; Pei-Kuen Wei

doi:10.1117/1.3116710

1 March 2009 Study of gold nanoparticles and live cells interactions by using planar evanescent wave excitation

Chia-Wei Lee, En-Hong Lin, Ji-Yen Cheng, Pei-Kuen Wei

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Journal of Biomedical Optics, Vol. 14, Issue 2, 021005 (March 2009). https://doi.org/10.1117/1.3116710

Abstract

We present a planar evanescent wave (PEW) technique combined with phase contrast optical microscopy to study the interactions between cells and gold nanoparticles (AuNPs). The PEW method employs a dual-fiber-line guide to couple light into a thin glass slide. It produces a uniform and long evanescent wave near the glass surface, as verified by the optical near-field measurement. High-contrast AuNP images are obtained by the PEW illumination. At the same time, cells are observed only by using the phase contrast microscopy. The nanoparticles and cell images indicated that unmodified AuNPs had no interactions with cells, possibly due to the negative surface charges on both cells and nanoparticles. The electrostatic concept was further verified by coating AuNPs with positively charged poly (L-lysine). DNA aptamers for surface mucin glycoprotein were coated on AuNPs to demonstrate the application for single nanoparticle tracking.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Chia-Wei Lee, En-Hong Lin, Ji-Yen Cheng, and Pei-Kuen Wei "Study of gold nanoparticles and live cells interactions by using planar evanescent wave excitation," Journal of Biomedical Optics 14(2), 021005 (1 March 2009). https://doi.org/10.1117/1.3116710

Published: 1 March 2009

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