Infrared spectroscopy reveals both qualitative and quantitative differences in equine subchondral bone during maturation

Yevgeniya Kobrina; Hanna Isaksson; Miikka Sinisaari; Lassi L. Rieppo; Pieter A. Brama; Rene van Weeren; Heikki J. Helminen; Jukka S. Jurvelin; Simo Saarakkala

doi:10.1117/1.3512177

1 November 2010 Infrared spectroscopy reveals both qualitative and quantitative differences in equine subchondral bone during maturation

Yevgeniya Kobrina, Hanna Isaksson, Miikka Sinisaari, Lassi L. Rieppo, Pieter A. Brama, Rene van Weeren, Heikki J. Helminen, Jukka S. Jurvelin, Simo Saarakkala

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Journal of Biomedical Optics, Vol. 15, Issue 6, 067003 (November 2010). https://doi.org/10.1117/1.3512177

Abstract

The collagen phase in bone is known to undergo major changes during growth and maturation. The objective of this study is to clarify whether Fourier transform infrared (FTIR) microspectroscopy, coupled with cluster analysis, can detect quantitative and qualitative changes in the collagen matrix of subchondral bone in horses during maturation and growth. Equine subchondral bone samples (n = 29) from the proximal joint surface of the first phalanx are prepared from two sites subjected to different loading conditions. Three age groups are studied: newborn (0 days old), immature (5 to 11 months old), and adult (6 to 10 years old) horses. Spatial collagen content and collagen cross-link ratio are quantified from the spectra. Additionally, normalized second derivative spectra of samples are clustered using the k-means clustering algorithm. In quantitative analysis, collagen content in the subchondral bone increases rapidly between the newborn and immature horses. The collagen cross-link ratio increases significantly with age. In qualitative analysis, clustering is able to separate newborn and adult samples into two different groups. The immature samples display some nonhomogeneity. In conclusion, this is the first study showing that FTIR spectral imaging combined with clustering techniques can detect quantitative and qualitative changes in the collagen matrix of subchondral bone during growth and maturation.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Yevgeniya Kobrina, Hanna Isaksson, Miikka Sinisaari, Lassi L. Rieppo, Pieter A. Brama, Rene van Weeren, Heikki J. Helminen, Jukka S. Jurvelin, and Simo Saarakkala "Infrared spectroscopy reveals both qualitative and quantitative differences in equine subchondral bone during maturation," Journal of Biomedical Optics 15(6), 067003 (1 November 2010). https://doi.org/10.1117/1.3512177

Published: 1 November 2010

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