Label-free optical activation of astrocyte in vivo

Myunghwan Choi; Taeyun Ku M.D.; Kyungsun Choi; Chulhee Choi; Jonghee Yoon

doi:10.1117/1.3600774

1 July 2011 Label-free optical activation of astrocyte in vivo

Myunghwan Choi, Taeyun Ku M.D., Kyungsun Choi, Chulhee Choi, Jonghee Yoon

Author Affiliations +

Journal of Biomedical Optics, Vol. 16, Issue 7, 075003 (July 2011). https://doi.org/10.1117/1.3600774

Abstract

As the most abundant cell type in the central nervous system, astrocyte has been one of main research topics in neuroscience. Although various tools have been developed, at present, there is no tool that allows noninvasive activation of astrocyte in vivo without genetic or pharmacological perturbation. Here we report a noninvasive label-free optical method for physiological astrocyte activation in vivo using a femtosecond pulsed laser. We showed the laser stimulation robustly induced astrocytic calcium activation in vivo and further verified physiological relevance of the calcium increase by demonstrating astrocyte mediated vasodilation in the brain. This novel optical method will facilitate noninvasive physiological study on astrocyte function.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Myunghwan Choi, Taeyun Ku M.D., Kyungsun Choi, Chulhee Choi, and Jonghee Yoon "Label-free optical activation of astrocyte in vivo," Journal of Biomedical Optics 16(7), 075003 (1 July 2011). https://doi.org/10.1117/1.3600774

Published: 1 July 2011

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY