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19 January 2012 Emerging concepts of laser-activated nanoparticles for tissue bonding
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We report recent achievements and future perspectives of minimally invasive bonding of biological tissues triggered by laser light. In particular, we review new advancements in the biomedical exploitation of near-infrared absorbing gold nanoparticles as an original solution for the photothermal closure of surgical incisions. Advanced concepts of laser tissue bonding involving the application of hybrid nanocomposites obtained by inclusion of nanochromophores into biopolymer scaffolds are also introduced. The perspectives of tissue bonding are discussed in the following aspects: (1) tissue bonding with highly-stabilized nanochromophores, (2) enhanced tissue bonding with patterned nanocomposites, (3) real-time monitoring of temperature distributions, (4) tracking of tissue regeneration based on the optical resonances of gold nanoparticles.


Laser Tissue Bonding



The development of minimally invasive techniques holding the promise of reduced tissue pain and faster recovery is one of the leading trends in surgical medicine. In particular, the possibility to perform surgeries without sutures or staples is a valuable objective in view of minimally invasive clinical interventions.1,2 One innovative approach for sutureless bonding entails the combination of laser light and an endogenous or exogenous optical absorber that may lie at the wound edges and mediate local generation of heat.3 The most relevant endogenous absorbers are water, melanin, and haemoglobin, which may be excited by infrared and visible light. In order to enhance the localization of light deposition, it proves advantageous to use an exogenous chromophore and light at frequencies overlapping its optical extinction bands within a window where the competition from the endogenous absorbers is poor.1,2 Finally, the thermal modification of the tissue is intended to generate new bonds between the wound edges, thus resulting in its closure.3


Current Laser Tissue Bonding Strategies

Nowadays, one of the preferred setups for sutureless laser-induced bonding involves the combination of near-infrared (NIR) light, which penetrates deep into the body, and an organic dye with optical extinction at the same frequencies.3 Among the NIR-absorbing dyes proposed for laser bonding, Indocyanine Green (ICG)4 has attracted very much interest from the medical community and is currently used to support a variety of surgical applications with a history of safety in humans.5,6 In a typical laser-welding procedure, an aqueous solution of ICG is accurately spread between the tissue margins to be welded, while paying particular care to leave surrounding tissues unstained, thus avoiding their accidental absorption of laser light.7 Then the wound edges are approximated and laser welding is performed under a surgical microscope by means of a NIR-emitting laser device (such as a diode laser) usually equipped with a fiberoptic delivery system. The heat produced upon irradiation with a NIR laser drives a thermal reorganization of the main tissue components, which results into the immediate closure and prompt repair of the wound.3,810 This strategy proves particularly appropriate for those applications where suturing and stapling may prevent a regular healing process due to foreign-body reaction, such as, for example, in lamellar or penetrating keratoplasty, or for the treatment of thin tissues such as the lens capsular bag.1113 Additional advantages include reduced operation times and a substantial simplification of the overall surgery. The above procedure may be customized for specific applications by varying the irradiation conditions, such as laser power, emission mode, treatment duration, and mutual arrangement between the fiber tip and tissue.14

In addition, different and functional formulations of ICG may be employed, including liquid, semisolid, and solid solutions.1,2 For instance, the chromophore may be homogeneously blended with a protein or polymer dispersion (e.g., albumin, fibrin, collagen, hyaluronan, etc.) that is pasted between the margins of the wound and finally irradiated with a NIR laser.1519 On heating, the ICG formulation coagulates and seals the wound, thus acting as a solder.20 When the adhesion is mediated by the topical interposition of soldering material, this bonding technique is more properly referred to as laser soldering.2 While liquid solders generally suffer from a high mobility of the absorbing chromophore during the soldering procedure, solid formulations exhibit low pliability, both of which hamper the achievement of reproducible results.2,21 In this context, the use of semisolid polymer formulations such as polycaprolactone (PCL) and chitosan in the form of flexible films and scaffolds is one hopeful alternative.18,2123 These materials, in addition to an intrinsic versatility in their fabrication process, hold the promise of good biocompatibility, biodegradability on a timescale of several days to months inside the body, and capacity to host functional chemicals, which attracts much interest for tissue repair and drug delivery purposes.24,25 Moreover, some formulations exhibit antimicrobial and chemoactractant properties, which can support and accelerate the wound healing process.26 In a recently proposed system, ICG molecules were loaded into silica nanoparticles, which are further dispersed with PCL and used in the form of semidry scaffolds.27 This system proved advantageous in improving the control over the local dye concentration, which is important to ensure better heat dosimetry.


Novel Tissue Bonding Strategies with Metal Nanochromophores

Despite the thorough research work that has been performed by several international groups in ex vivo models and in in vivo animal models,2,11,28,29 only a few of the proposed approaches have reached the preclinical and clinical phases thus far.11,12,30,31 The reason for this gap has to be found in a lack of standardization in the tissue bonding procedure, mainly related to the use of conventional organic chromophores and, in turn, to a poor control over the heat deposition, as mentioned above.

Notable issues ascribed to the use of exogenous chromophores of common use in surgical practices such as ICG include a dependence of their structural and optical response on their biochemical environment and temperature, and a rapid degradation over time.4,3234 In addition, these molecules suffer from limited light extinction efficiency, photobleaching, and possible phototoxicity (e.g., generation of reactive oxygen species).4,35 With this background, there have been conceived recent proposals to replace these organic chromophores by metal36 and composite metal-coated bead37 nanoscale transducers, which hold the promise to extend the range of application of tissue bonding.21,38 Similar to the organic dyes, these nanoparticles can be formulated both in aqueous suspensions and after dispersion in a suitable protein or biopolymer scaffold.3,21 In the latter case, the biochemical matrix is activated by the photothermal conversion from the embedded chromophores, ultimately adhering to its adjacent tissues.1

To the broad class of metal nanoparticles belong the so-called gold nanorods (cylindrical nanoparticles) and gold nanoshells (silica core, gold shell), which are the most efficient nanochromophores for photothermal applications in biomedicine disclosed thus far.39,40 Typical extinction spectra of gold nanorods and gold nanoshells display characteristic features originating from collective electron oscillations or plasmon resonances and comprise a weaker band in the green (such as that found for gold nanospheres) and a fairly stronger band in the NIR.4143 The latter can be tuned throughout the NIR window in a controlled manner during the nanoparticles fabrication, by changing the aspect ratio (length divided by waist diameter) of gold nanorods or the core diameter to shell thickness ratio of gold nanoshells (Fig. 1).40,4345

Fig. 1

(a) Gold nanorods of different aspect ratios display different colors which correspond to different absorption spectra (bar=100nm). Reproduced with permission from Ref. 46. (b) Optical tunability for nanoshells with a 60-nm silica core radius and gold shells 5, 7, 10, and 20 nm thick. The plasmon resonance red shifts with decreasing thickness of the gold shell (or increasing core to shell ratio). Reproduced with permission from Ref. 43.


In turn, upon excitation with radiation from a NIR laser, these plasmon resonances undergo excitation and relaxation mainly through nonradiative channels. The interplay of physical and chemical properties of gold nanorods and gold nanoshells gives a number of favourable features,21,40,41,4649 such as: (1) possibility to modulate the geometrical and optical properties during fabrication; (2) a unique enhancement of the near field; (3) exceptional absorption (e.g., gold nanorods can provide absorption coefficients higher by up to five orders of magnitude than those of conventional absorbing dyes) and scattering (preferentially ascribed to gold nanoshells) efficiencies; (4) high stability in the body and thresholds before photothermal degradation; (5) broad versatility of conjugation with additional biochemical molecules including ligands, drugs, and genes; and (6) inertness in the body and negligible intrinsic cytotoxicity. For these reasons, these nanoparticles have been investigated as valuable contrast agents and sensitizers in a variety of diagnostic (e.g., optical and photoacoustic imaging and optical coherence tomography) and therapeutic (e.g., photothermal and photoacoustic microsurgery) applications50,51 including laser bonding.

A few papers reporting the successful application of gold nanorods and gold nanoshells for tissue bonding have appeared in the recent scientific literature. For instance, Gobin et al. have demonstrated the use of a solder composed of bovine serum albumin (BSA) and gold nanoshells (110nm diameter and 10nm shell thickness) to successfully seal ex vivo muscular and in vivo skin tissue lesions.37 These authors used continuous wave radiation generated by a diode laser emitting at 808 to 820 nm with a 14 to 20Wcm2 intensity to achieve successful fusion with tensile strength equal to that of native tissues. Similarly, Nourbakhsh and Khosroshahi52 realized the closure of 20-mm-long incisions performed on explanted skin samples mounted on an automatic programmable scanning system by using a diode laser (peak emission at 810 nm) running in continuous wave mode at an optimal intensity of 60Wcm2. Recently, our research group demonstrated the successful application of an aqueous suspension of gold nanorods for the direct welding of explanted lens capsular tissues.53 Later substantial advancements have been obtained upon inclusion of gold nanorods into polysaccharide scaffolds.21 In these systems the sugar chains enwrap the nanoparticles, thus creating a protective barrier against the physiological environment and enhancing their stability, durability, effectiveness, and biocompatibility. Preliminary investigations consisted of in vivo laser closuring of full thickness incisions performed in the carotid artery wall by means of a 810-nm diode laser in conjunction with the topical application of a composite gel of hyaluronan and gold nanorods (Fig. 2).54 Irradiation of the wounds with a continuous intensity of 40Wcm2 provided successful closure of their edges with sufficient strength to sustain blood pressure and ideal healing within a 30-day follow-up.

Fig. 2

Sequence of images recorded during the laser welding of a rabbit carotid artery with a gold nanorods/hyaluronan gel. (a) The artery is clamped and a 3-mm longitudinal incision is performed. (b) After application of the soldering formulation, the incision is treated with a 810-nm diode laser light. (c) Appearance of the sealed artery immediately after intervention.


A further optimization of tissue repair procedures with gold nanochromophores consisted of the use of flexible materials with enhanced handiness and stability with respect to liquid or soluble solders such as those mentioned above. We recently proposed the introduction of a novel biocompatible material consisting of chitosan films containing gold nanorods, which can be readily bonded to biological tissues by photothermal activation.55 These films are resistant, pliable, and stable in physiological conditions over time, while the chitosan matrix provides excellent control over the distribution and stability against aggregation of the nanoparticles, which translates into a dependable optical response and photothermal conversion [Figs. 3(a) and 3(b)]. Effective and reproducible adhesion of these films onto explanted carotid artery samples was achieved by means of contiguous spots of laser irradiation realized by gently bringing an optical fiber tip into contact with the films and delivering 810-nm diode laser pulses of 100 ms duration and 100 mJ energy, corresponding to 140Jcm2 fluence at the outermost film surface [Figs. 3(c)3(f)].55

Fig. 3

(a) Comparison between the light extinction profiles of a hybrid gold/chitosan film (solid line) and the initial aqueous suspension of gold nanorods (dashed line). (b) SEM image of the gold nanorods distribution within a film sample (bar=50μm). A good balance between gold nanorods and chitosan concentrations provided for a homogeneous dispersion of particles within the polymer medium with an optical absorption profile closely resembling that of isolated nanoparticles. (c) Scheme of laser bonding of a hybrid gold/chitosan film to an arterial tissue—1: the artery is clamped proximally and distally and a 5-mm longitudinal cut is performed by means of a sharp-point; 2 and 3: a hybrid gold/chitosan film is topically applied onto the lesion and rolled all over the artery and 4: laser welded into its final position. Laser bonding is obtained by keeping the fiber tip in contact with the outer layer of the film and delivering single laser pulses of 100 mJ, 100 ms. (d) Toluidine blue stain of a cross-sectional slice of a film/tissue adhesion (bar=100μm). Comparison between TEM images of an untreated hybrid gold/chitosan film region (e) and of an adhesion region at the tissue/film interface (f) (bar=500nm; f=film; t=arterialtissue; gold nanorods are evidenced by arrows). A homogeneous adhesion characterized by close interdigitation between the biopolymer matrix and the external arterial wall is evidenced.


Although beyond the focus of this paper, it is worth mentioning a different nanoparticle-inspired tissue bonding approach, which is based on magnetic instead of laser stimulation. This was recently proposed by Bregy et al.56 with the final aim of reducing the effects of the inhomogeneities in the solder absorption and of increasing the radiation penetration through the tissue. The use of an albumin solder enriched with spherical superparamagnetic iron oxide nanoparticles (SPIONs) of 15 nm diameter allowed these authors to weld explanted blood vessels on application of 170-m radio waves56 with strength comparable to that obtained with conventional suturing.


Possible Developments and Perspectives


Tissue Bonding with Highly Stabilized Gold Nanochromophores

The positive reception of gold nanochromophores for important photothermal applications, such as the laser hyperthermia of cancer, is backed by their excellent photostability, which outclasses that of organic dyes. However, tissue bonding may require unusually high laser fluences (well above 10Wcm2), which may challenge the shape durability of these nanoparticles. For instance, already well below the melting point of bulk gold, gold nanorods tend to transform into stable gold nanospheres on laser excitation or thermal bathing,57,58 which may result from surface diffusion59,60 and immediately translate into a loss of plasmon resonances and light extinction in the NIR window. Figure 4(a) displays the appearance of a chitosan film loaded with gold nanorods and irradiated through its central spot under conditions that may be required for tissue bonding. Already after a few seconds, this spot is seen to undergo a visible change in color from purple to green, which reflects the bleaching of the gold nanochromophores (i.e., their inhibition to further laser absorption). Similar dynamics have been reported for the gold layer surrounding the silica core of gold nanoshells as well, which was shown to degrade into byproducts such as small gold nanospheres with a large size distribution and little capacity to absorb NIR light.61

Fig. 4

(a) A1cm diameter gold nanorods/chitosan film after exposure to 18Wcm2 810 nm laser light, which is typical for laser bonding. Note the change in color at the 2-mm-diameter central spot under irradiation. (b) Representative TEM micrograph of silica-coated gold nanorods, which may benefit from additional stability.


A possible solution may come from surface modification of the particles with a rigid inorganic or organic coating, in an attempt to impart enhanced stability and a mechanical constraint against photothermal reshaping.41 For instance, deposition of a silica shell around gold nanorods [e.g., Fig. 4(b)] was shown to substantially delay the deformation of gold nanorods in front of NIR laser pulses in the second-to-nanosecond timescales.6264 Similarly, a carbon or polymethylmethacrylate coating was reported to significantly improve the photostability of gold nanoparticles.65,66 The possibility to sustain high local temperatures is expected to enable better stability of the photothermal conversion and thus increase the overall reproducibility of tissue bonding. In addition, highly stable nanochromophores shall be less prone to laser-induced decomposition into small fragments,66 whose presence may be undesirable in vivo. In fact, previous cytotoxicity studies provided evidence for cell death by necrosis or apoptosis when using clusters of few gold atoms around 1.4 nm in size,46,67 which become negligible for gold nanoparticles of larger size.46,6870


Enhanced Tissue Bonding with Patterned Nanocomposites

Further optimization of the tissue bonding technology may be achieved by the use of patterned nanocomposites. By this phrase we intend the fabrication of well-defined and functional particle geometries and distributions inside a composite formulation.71,72 One example of patterned nanocomposites includes matrices with a gradient of optical absorbance along their length. These shall find application as laser-activated inserts inside an accidental wound or surgical incision. Indeed, the deeper the nanochromophore inside the cut, the larger the absorbance that is needed in order to produce an effective photothermal response, which is due to the light attenuation from the upper layers.73 While the fabrication of durable graduated inserts with organic dyes remains a challenge,23 this may be achieved for instance by subjecting gold nanorods embedded into a polymer strip to a suitable temperature gradient in order to modulate the particle’s transformation along its length and so the spectrum of plasmon resonances.65


Real-Time Temperature Monitoring by Optoacoustic Tomography during Tissue Bonding

Subtle temperature dynamics inside the tissue critically affect the closure of a wound.7476 Indeed, the temperature increase needs to be high enough to trigger particular thermal processes2,3 yet without inducing irreversible thermal damage, with a narrow window of optimal conditions.77,78 Nowadays commercially available systems that enable a real-time, noninvasive monitoring of temperature dynamics in the clinical practice are based on infrared detectors such as thermocameras, which provide the spatial distribution and temporal evolution of temperature with very decent resolution. Unfortunately, this approach only provides a superficial temperature detection, which prevents fundamental insight into the temperature distribution inside the target. To overcome this restriction, analytical models based on the solution of the bioheat equation in a two- or three-dimensional domain are used to approximately track the temperature evolution deep inside the tissue.3,75 However, use of these models is very delicate, due to the great complexity of biological tissues with their heterogeneous composition and subtle dissipative pathways. Valuable alternatives are represented by magnetic resonance imaging (MRI)79 and ultrasound imaging,80 which provide for a direct real-time monitoring of the temperature rise within biological tissues subject to photothermal treatments without the need for numerical postprocessing. However, while MRI does not hold much promise to become a routine tool for monitoring thermal effects deep in the body,81,82 efficient equipment for ultrasonic detection of temperature in the clinical practice is not yet available.83

A recent and powerful alternative is nowadays represented by optoacoustic tomography (OAT), also called photoacoustic tomography (PAT), which is based on photoacoustic conversion (i.e., the generation of pressure waves and emission of ultrasound on absorption of light energy, preferably under thermal and stress confinement conditions).84 OAT/PAT combines optical contrast based on absorption of NIR optical energy and detection of resulting acoustic pressure propagating as ultrasonic waves, resulting in a much higher lateral and depth resolution than pure ultrasonography and higher sensitivity with respect to MRI.8587 Due to the inherent optical contrast between cancerous and healthy tissue, OAT/PAT has originally found application in identifying cancerous lesions.8890 More recently, OAT/PAT has been proposed to measure temperature profiles by tracking thermally induced changes in thermoelastic properties and photoacoustic signal amplitudes (Fig. 5).9194 In this context, gold nanoparticles have been proposed as effective contrast agents for image-guided therapy based on photoacoustic imaging.90,95 Once irradiated with pulsed laser light at low average power, these particles act as very local heat sources with temperature rapidly fading away with distance,96 thus capable of triggering the photoacoustic conversion without interfering much with the overall tissue temperature. At present OAT/PAT represents a promising candidate to precisely monitor temperature changes during chromophore-enhanced photothermal therapies.91 We anticipate a future involvement of OAT/PAT in the real-time guiding of photothermal tissue bonding with nanochromophores or nanocomposites. The possibility to simultaneously implement both techniques by the use of the same particles and with an endoscope will provide further benefits for extreme surgeries including the laser welding of gastrointestinal, urological, or intracranial tracts.

Fig. 5

(a) Photoacoustic (PA) signal and actual temperature of a source sample (diluted black ink solution with μa=30cm1) as the heated sample was allowed to come to room temperature. The linear relationship between temperature and PA signal proves potential for real-time temperature sensing, (b) PA signal versus temperature from (a). The green line is a linear fit with an R2 of 0.98. (c) PA signal and actual temperature of an initially cold sample warming up to room temperature, (d) PA signal versus temperature from (c). Again, the green line is a linear fit with an R2 of 0.98. Reproduced with permission from Ref. 92.



Tracking of Tissue Regeneration Based on the Optical Resonances of Gold Particles

A key concept to take full advantage of gold nanoparticles for tissue bonding is the tunability of their optical properties, as it was already mentioned in Sec. 1.3 and Fig. 1, which makes these new chromophores particularly attractive for theranostic applications.97 In order to expand this point, Fig. 6 displays a collection of simulations of the light absorption (uppermost curves) and scattering (lowermost curves) cross sections for prolate elliptical gold nanoparticles in water with parameters representative of typical gold nanorods. As is shown, the two contributions of light extinction (scattering and absorption) of the particles can be modulated in amplitude and position throughout the visible NIR region by changing their size (volume) and shape (aspect ratio). As a rule of thumb, smaller nanoparticles are ideal for OAT/PAT and photothermal treatment procedures as light absorption dominates, while larger nanoparticles exhibit higher scattering efficiency, which may be preferable for specific optical imaging applications including optical coherence tomography (OCT).

Fig. 6

Simulations obtained from Gans dipolar approximation,98100 and implemented with the analytical gold dielectric function given by Etchegoin et al.101, which describe the light absorption (upper curves) and scattering (lower curves) cross sections from prolate elliptical gold nanoparticles with different shape (a) and size (b). (a) Variation of the optical properties of gold nanoparticles with the same volume V=1000nm3 and different length/diameter ratios (i.e., aspect ratio [AR]) from 2.5 to 5 in steps of 0.5 from left to right. The plasmon band in the NIR window progressively gains intensity and red-shifts with AR. (b) Effect of a constant AR=4 and different volumes from 1000nm3 to 5000nm3 in steps of 1000nm3 from the low to the high scattering bands (the absorption bands scale with V in Gans models and so the absorption profiles overlap with each other in the normalization given in this figure). The principal bias is an increase of the ratio of light scattering to absorption with volume.


OCT provides noninvasive imaging of living tissues with penetration depth in the low millimeter range and spatial resolution in the low micron range, which is superior to standard clinical methods of noninvasive imaging such as ultrasonography and MRI.102 Highly scattering gold nanoshells (119 nm diameter, 12 nm shell thickness) have been shown to enhance OCT contrast in vivo.51 In this example, both imaging and photothermal therapy have been performed with a single particle formulation designed for both scattering and absorption.103 Different OCT modalities are based on differential absorption or backscattering albedo (the ratio of backscattering to total extinction), which offer the advantage of producing contrast in tissues with high turbidity.104 In this context it is the absorption properties of plasmonic nanoparticles that are concerned and should prevail in comparison with the case of the conventional backscattering OCT modality.

The twofold functionality of gold nanoparticles as both photothermal transducers to trigger tissue repair and contrast labels for biomedical diagnosis is well suited to provide for breakthrough insight into tissue bonding applications. Resonant imaging based on gold nanoparticles dispersed at the weld site will be useful to track the progress of the wound healing process in the period that follows the tissue bonding intervention. The low diffusivity of gold particles through the tissue matrix with respect to that of organic chromophores (e.g., 108cm2s1 for 100-nm gold nanoparticles105 as compared with 106cm2s1 for ICG106 through connective tissues) implies a long-term permanence of the former in situ, while the latter are cleaned from the body within two to three days, which generates intense interest for their use for tissue monitoring during the follow-up period. This tendency can be amplified by increasing the nanoparticles size.105 Previous studies have demonstrated the presence of 40-nm gold nanoparticles interdispersed within the extracellular matrix up to at least 30 days after a tissue bonding intervention (Fig. 7).21,54 In addition, the use of functionalized particles capable of labeling different cell-surface factors or extracellular matrix biomarkers is expected to confer specificity to a particular biophysical or biochemical response occurring during tissue regeneration such as fibroblast migration, collagen deposition, and self-organization, and provide information on the evolution of these processes.107,108

Fig. 7

TEM micrograph (4.2×3.0μm2) of a closured rabbit carotid artery after a 30-day follow up from laser bonding with gold nanochromophores.




Recent progress in the design and synthesis of novel nanoparticles with versatile optical response holds much potential to improve the current technology of tissue bonding based on organic dyes and extend its range of application. In this context, the combination of NIR-absorbing gold nanoparticles with a diode laser irradiation matching their plasmon absorption resonance represents an original solution. A further technical development involves the preparation of hybrid adhesives by dispersion of the nanochromophores into a biopolymer matrix in order to achieve stable laser-activatable nanocomposites, which provide for reproducible and resistant adhesion.

Possible future scenarios of successful bonding of biological tissues include engineering of nanochromophores with enhanced resistance against photothermal reshaping in order to preserve their characteristic plasmon resonances against high laser fluences, and of patterned nanocomposites with well-defined particle geometries, which may provide for thorough control over the laser excitation. In addition, the photoacoustic signal generated by gold nanoparticles at the weld site may be exploited to perform a real-time monitoring of local tissue temperature for guided photothermal tissue bonding. Another key concept to take full advantage of gold nanoparticles for tissue repair is the tunability of their optical response, which may enable resonant imaging based on different modalities (OAT/PAT and OCT) to track tissue regeneration during the follow-up period.

While gold nanoparticle—mediated photothermal bonding is at its very early stages, recent advancements in biomedical optics and nanomedicine let us expect a rapid development of this technology. Additional optimization strategies such as those discussed above are needed in order to provide for full reproducibility and control over the photothermal process and tissue regeneration and ultimately build a standardized nanophotonics-based bonding procedure and devise surgeon-friendly protocols that may find application in the clinical practice.


This work has been partially supported by the Projects of the Health Board of the Tuscany Region “NANO TREAT” and “NANO-CHROM” and by the Network of Excellence “Photonics 4 Life.”



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© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
Paolo Matteini, Fulvio Ratto, Francesca Rossi, and Roberto Pini "Emerging concepts of laser-activated nanoparticles for tissue bonding," Journal of Biomedical Optics 17(1), 010701 (19 January 2012).
Published: 19 January 2012

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