Near-infrared fluorescence imaging of experimentally collagen-induced arthritis in rats using the nonspecific dye tetrasulfocyanine in comparison with gadolinium-based contrast-enhanced magnetic resonance imaging, histology, and clinical score

Ines Gemeinhardt; Dorothee Puls; Ole Gemeinhardt; Matthias Taupitz; Susanne Wagner; Beatrix Schnorr; Kai Licha; Michael Schirner; Bernd Ebert; Diethard Petzelt; Rainer Macdonald; Jörg Schnorr

doi:10.1117/1.JBO.17.10.106008

1 October 2012 Near-infrared fluorescence imaging of experimentally collagen-induced arthritis in rats using the nonspecific dye tetrasulfocyanine in comparison with gadolinium-based contrast-enhanced magnetic resonance imaging, histology, and clinical score

Ines Gemeinhardt, Dorothee Puls, Ole Gemeinhardt, Matthias Taupitz, Susanne Wagner, Beatrix Schnorr, Kai Licha, Michael Schirner, Bernd Ebert, Diethard Petzelt, Rainer Macdonald, Jörg Schnorr

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Journal of Biomedical Optics, Vol. 17, Issue 10, 106008 (October 2012). https://doi.org/10.1117/1.JBO.17.10.106008

Abstract

Using 15 rats with collagen-induced arthritis (30 joints) and 7 control rats (14 joints), we correlated the intensity of near-infrared fluorescence (NIRF) of the nonspecific dye tetrasulfocyanine (TSC) with magnetic resonance imaging (MRI), histopathology, and clinical score. Fluorescence images were obtained in reflection geometry using a NIRF camera system. Normalized fluorescence intensity (I NF ) was determined after intravenous dye administration on different time points up to 120 min. Contrast-enhanced MRI using gadodiamide was performed after NIRF imaging. Analyses were performed in a blinded fashion. Histopathological and clinical scores were determined for each ankle joint. I NF of moderate and high-grade arthritic joints were significantly higher (p<0.005 ) than the values of control and low-grade arthritic joints between 5 and 30 min after TSC-injection. This result correlated well with post-contrast MRI signal intensities at about 5 min after gadodiamide administration. Furthermore, I NF and signal increase on contrast-enhanced MRI showed high correlation with clinical and histopathological scores. Sensitivities and specificities for detection of moderate and high-grade arthritic joints were slightly lower for NIRF imaging (89%/81% ) than for MRI (100%/91% ). NIRF imaging using TSC, which is characterized by slower plasma clearance compared to indocyanine green (ICG), has the potential to improve monitoring of inflamed joints.

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Ines Gemeinhardt, Dorothee Puls, Ole Gemeinhardt, Matthias Taupitz, Susanne Wagner, Beatrix Schnorr, Kai Licha, Michael Schirner, Bernd Ebert, Diethard Petzelt, Rainer Macdonald, and Jörg Schnorr "Near-infrared fluorescence imaging of experimentally collagen-induced arthritis in rats using the nonspecific dye tetrasulfocyanine in comparison with gadolinium-based contrast-enhanced magnetic resonance imaging, histology, and clinical score," Journal of Biomedical Optics 17(10), 106008 (1 October 2012). https://doi.org/10.1117/1.JBO.17.10.106008

Published: 1 October 2012

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