8 August 2013 Errata: High-sensitivity detection of breast tumors in vivo by use of a pH-sensitive near-infrared fluorescence probe
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Abstract
This article [J. Biomed. Opt.. 17, , 076028 (2012)] was originally published online on 27 July 2012 with Figures 1 and 3 reversed. The corrected figures are reprinted below.

This article [J. Biomed. Opt. 17, 076028 (2012)] was originally published online on 27 July 2012 with Figures 1 and 3 reversed. The corrected figures are reprinted below.

Fig. 1

Fluorescence emission spectra of probes at different pH. Representative uncorrected fluorescence emission spectra (n=3) of pH-Her (a) and Alexa-Her (b) measured in PBS at pH of 7.5 (black curve) and of 5.5 (red dashed curve) shown as examples for conjugates with a DP ratio of 1.6 (n=3); excitation was at λex 635 nm.

JBO_18_8_089801_f001.png

Fig. 3

Fluorescence microscopy demonstrates internalization-dependent activation of pH-Her. Breast cancer cells grown on culture slides were incubated for 8 h with pH-Her or Alexa-Her. On the left panel, counterstain of cell nuclei with Hoechst 33342, in the middle, probe-derived signals, and on the right panel, merged images of the cell nuclei (blue) and the probe (red) are illustrated. (a), When incubated with KPL-4 cells at 37°C, pH-Her shows fluorescence only after receptor-mediated internalization (green arrow). (b), At 4°C, no signals from the pH-sensitive probe presumably bound to the cell membrane can be detected. (c), Alexa-Her shows fluorescence from the internalized probe (green arrow) and also membrane-derived fluorescence can be observed after 8 h of incubation at 37°C (c) and 4°C (d) (orange arrow, no internalization). Representative images of three independently performed experiments are presented. Bars represent 50 μm.

JBO_18_8_089801_f003.png

This article was corrected online on 6 August 2013.

© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Julia E. Mathejczyk, Julia E. Mathejczyk, Jutta Pauli, Jutta Pauli, Christian Dullin, Christian Dullin, Ute Resch-Genger, Ute Resch-Genger, Frauke Alves, Frauke Alves, Joanna Napp, Joanna Napp, } "Errata: High-sensitivity detection of breast tumors in vivo by use of a pH-sensitive near-infrared fluorescence probe," Journal of Biomedical Optics 18(8), 089801 (8 August 2013). https://doi.org/10.1117/1.JBO.18.8.089801 . Submission:
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