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16 December 2013 Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy
Zufang Huang, Yan Sun, Jing Wang, Shengrong Du, Yongzeng Li, Juqiang Lin, Shangyuan Feng, Jinping Lei, Hongxin Lin, Rong Chen, Haishan Zeng
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Journal of Biomedical Optics, Vol. 18, Issue 12, 127003 (December 2013). https://doi.org/10.1117/1.JBO.18.12.127003
Abstract
In this study, a rapid and simple method which combines drop coating deposition and Raman spectroscopy (DCDR) was developed to characterize the dry embryo culture media (ECM) droplet. We demonstrated that Raman spectra obtained from the droplet edge presented useful and characteristic signatures for protein and amino acids assessment. Using a different analytical method, scanning electron microscopy coupled with energy dispersive X-ray analysis, we further confirmed that Na, K, and Cl were mainly detected in the central area of the dry ECM droplet while sulphur, an indicative of the presence of macromolecules such as proteins, was mainly found at the periphery of the droplet. In addition, to reduce sample preparation time, different temperatures for drying the droplets were tested. The results showed that drying temperature at 50°C can effectively reduce the sample preparation time to 6 min (as compared to 50 min for drying at room temperature, ∼25°C ) without inducing thermal damage to the proteins. This work demonstrated that DCDR has potential for rapid and reliable metabolomic profiling of ECM in clinical applications.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Zufang Huang, Yan Sun, Jing Wang, Shengrong Du, Yongzeng Li, Juqiang Lin, Shangyuan Feng, Jinping Lei, Hongxin Lin, Rong Chen, and Haishan Zeng "Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy," Journal of Biomedical Optics 18(12), 127003 (16 December 2013). https://doi.org/10.1117/1.JBO.18.12.127003
Published: 16 December 2013
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Cited by 10 scholarly publications.
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KEYWORDS
Raman spectroscopy
Proteins
Nondestructive evaluation
Coating
Scanning electron microscopy
Profiling
X-rays
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