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3 October 2014 Different doses of low-level laser irradiation modulate the in vitro response of osteoblast-like cells
Serena Incerti Parenti, Luigi Checchi, Milena Fini, Matilde Tschon
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Journal of Biomedical Optics, Vol. 19, Issue 10, 108002 (October 2014). https://doi.org/10.1117/1.JBO.19.10.108002
Abstract
Because osteoblasts play a key role in bone remodeling and the influence of low-level laser therapy on this process is not clear, Saos-2 human osteoblast-like cells were irradiated by a gallium–aluminum–arsenide diode laser (915 nm) for 10, 48, 96, 193, and 482 s using doses 1, 5, 10, 20, and 50  J/cm2, respectively. A control group was not irradiated. Morphology, viability, and cytotoxicity analyses were carried out after 1 hr, 1 day, and 3 days. Deoxyribose nucleic acid (DNA) content and release of vascular endothelial growth factor (VEGF), receptor activator of nuclear factor kappa B ligand (RANKL), and osteoprotegerin (OPG) were evaluated. Viability was modulated by laser irradiation in a dose-dependent manner, with 10  J/cm2 inducing a biostimulatory response and 20 to 50  J/cm2 determining a bioinhibitory and cytotoxic effect. Accordingly, DNA content was generally increased for the 10  J/cm2 dose and decreased for the 50  J/cm2 dose. A rapid and transitory trend toward increased RANKL/OPG ratio and a tendency toward a delayed increase in VEGF release for doses of 1 to 10  J/cm2 was found. Further investigations using the biostimulatory dose of 10  J/cm2 emerged from this study are needed to establish the ideal treatment regimens in the laboratory as well as in clinical practice.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2014/$25.00 © 2014 SPIE
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Serena Incerti Parenti, Luigi Checchi, Milena Fini, and Matilde Tschon "Different doses of low-level laser irradiation modulate the in vitro response of osteoblast-like cells," Journal of Biomedical Optics 19(10), 108002 (3 October 2014). https://doi.org/10.1117/1.JBO.19.10.108002
Published: 3 October 2014
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KEYWORDS
Laser irradiation
In vitro testing
Bone
Modulation
Semiconductor lasers
Receptors
Laser therapeutics
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