Cone-beam x-ray luminescence computed tomography (CB-XLCT), as an emerging imaging technique, plays an important role in in vivo small animal imaging studies. However, CB-XLCT suffers from low-spatial resolution due to the ill-posed nature of reconstruction. We improve the imaging performance of CB-XLCT by using a multiband excitation-resolved imaging scheme combined with principal component analysis. To evaluate the performance of the proposed method, the physical phantom experiment is performed with a custom-made XLCT/XCT imaging system. The experimental results validate the feasibility of the method, where two adjacent nanophosphors (with an edge-to-edge distance of 2.4 mm) can be located.
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The study was to investigate the changes in biochemical properties of activated mature CD8+ T cells related to apoptosis at a molecular level. We confirmed the activation and apoptosis of CD8+ T cells by fluorescence-activated cell sorting and atomic force microscopy and then performed Raman spectral measurements on activated mature CD8+ T cells and cellular deoxyribose nucleic acid (DNA). In the activated mature CD8+ T cells, there were increases in protein spectra at 1002 and 1234 cm−1. In particular, to assess the apoptosis-related DNA spectral signatures, we investigated the spectra of the cellular DNA isolated from resting and activated mature CD8+ T cells. Raman spectra at 765 to 786 cm−1 and 1053 to 1087 cm−1 were decreased in activated mature DNA. In addition, we analyzed Raman spectrum using the multivariate statistical method including principal component analysis. Raman spectra of activated mature DNA are especially well-discriminated from those of resting DNA. Our findings regarding the biochemical and structural changes associated with apoptosis in activated mature T cells and cellular DNA according to Raman spectroscopy provide important insights into allospecific immune responses generated after organ transplantation, and may be useful for therapeutic manipulation of the immune response.
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Our objective was to validate a new device dedicated to measure the light disturbances surrounding bright sources of light under different sources of potential variability. Twenty subjects were involved in the study. Light distortion was measured using an experimental prototype (light distortion analyzer, CEORLab, University of Minho, Portugal) comprising twenty-four LED arrays panel at 2 m. Sources of variability included: intrasession and intersession repeated measures, pupil size (3 versus 6 mm), defocus (+0.50) correction for the working distance, angular resolution (15 deg versus 30 deg), temporal stimuli presentation, and pupil size. Size, shape, location, and irregularity parameters have been obtained. At a low speed of presentation of the stimuli, changes in angular resolution did not have an effect on the results of the parameters measured. Results did not change with pupil size. Intensity of the central glare source significantly influenced the outcomes. Examination time was reduced by 30% when a 30 deg angular resolution was explored instead of 15 deg. Measurements were fast and repeatable under the same experimental conditions. Size and shape parameters showed the highest consistency, whereas location and irregularity parameters showed lower consistency. The system was sensitive to changes in the intensity of the central glare source but not to pupil changes in this sample of healthy subjects.
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A three-dimensional (3-D) point spread function (PSF) model for wide-field fluorescence microscopy, suitable for imaging samples with variable refractive index (RI) in multilayered media, is presented. This PSF model is a key component for accurate 3-D image restoration of thick biological samples, such as lung tissue. Microscope- and specimen-derived parameters are combined with a rigorous vectorial formulation to obtain a new PSF model that accounts for additional aberrations due to specimen RI variability. Experimental evaluation and verification of the PSF model was accomplished using images from 175-nm fluorescent beads in a controlled test sample. Fundamental experimental validation of the advantage of using improved PSFs in depth-variant restoration was accomplished by restoring experimental data from beads (6 μm in diameter) mounted in a sample with RI variation. In the investigated study, improvement in restoration accuracy in the range of 18 to 35% was observed when PSFs from the proposed model were used over restoration using PSFs from an existing model. The new PSF model was further validated by showing that its prediction compares to an experimental PSF (determined from 175-nm beads located below a thick rat lung slice) with a 42% improved accuracy over the current PSF model prediction.
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Photodynamic therapy (PDT) is currently used in the treatment of brain tumors. However, not only malignant cells but also neighboring normal neurons and glial cells are damaged during PDT. In order to study the potential role of transcription factors—nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), activator protein (AP-1), and signal transducer and activator of transcription-3 (STAT-3)—in photodynamic injury of normal neurons and glia, we photosensitized the isolated crayfish mechanoreceptor consisting of a single sensory neuron enveloped by glial cells. Application of different inhibitors and activators showed that transcription factors NF-κB (inhibitors caffeic acid phenethyl ester and parthenolide, activator betulinic acid), AP-1 (inhibitor SR11302), and STAT-3 (inhibitors stattic and cucurbitacine) influenced PDT-induced death and survival of neurons and glial cells in different ways. These experiments indicated involvement of NF-κB in PDT-induced necrosis of neurons and apoptosis of glial cells. However, in glial cells, it played the antinecrotic role. AP-1 was not involved in PDT-induced necrosis of neurons and glia, but mediated glial apoptosis. STAT-3 was involved in PDT-induced apoptosis of glial cells and necrosis of neurons and glia. Therefore, signaling pathways that regulate cell death and survival in neurons and glial cells are different. Using various inhibitors or activators of transcription factors, one can differently influence the sensitivity and resistance of neurons and glial cells to PDT.
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To better understand how the eye’s optics affects stereopsis, we measured stereoacuity before and after higher-order aberration (HOA) correction with a binocular adaptive optics visual simulator. The HOAs were corrected either binocularly or monocularly in the better eye (the eye with better contrast sensitivity). A two-line stereo pattern served as the visual stimulus. Stereo thresholds at different viewing durations were obtained with the psychophysical method of constant stimuli. Binocular HOA correction led to significant improvement in stereoacuity. However, better eye HOA correction could bring either a bad degradation or a slight improvement in stereoacuity. As viewing duration increased, the stereo benefit approached the level of 1.0 for both binocular and better eye correction, suggesting that long viewing durations might weaken the effects of the eye’s optical quality on stereopsis.
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Feasibility of speckle tracking in optical coherence tomography (OCT) based on digital image correlation (DIC) is discussed in the context of elastography problems. Specifics of applying DIC methods to OCT, compared to processing of photographic images in mechanical engineering applications, are emphasized and main complications are pointed out. Analytical arguments are augmented by accurate numerical simulations of OCT speckle patterns. In contrast to DIC processing for displacement and strain estimation in photographic images, the accuracy of correlational speckle tracking in deformed OCT images is strongly affected by the coherent nature of speckles, for which strain-induced complications of speckle “blinking” and “boiling” are typical. The tracking accuracy is further compromised by the usually more pronounced pixelated structure of OCT scans compared with digital photographic images in classical DIC applications. Processing of complex-valued OCT data (comprising both amplitude and phase) compared to intensity-only scans mitigates these deleterious effects to some degree. Criteria of the attainable speckle tracking accuracy and its dependence on the key OCT system parameters are established.
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Colorectal cancer is one of the most aggressive cancers usually occurring in people above the age of 50 years. In the United States, colorectal cancer is the third most diagnosed cancer. The American Cancer Society has estimated 96,830 new cases of colon cancer and 40,000 new cases of rectal cancer in 2014 in the United States. According to the literature, up to 55% of colorectal cancer patients experience a recurrence within five years from the time of surgery. Relapse of colorectal cancer has a deep influence on the quality of patient life. Infrared (IR) spectroscopy has been widely used in medicine. It is a noninvasive, nondestructive technique that can detect changes in cells and tissues that are caused by different disorders, such as cancer. Abnormalities in the colonic crypts, which are not detectable using standard histopathological methods, could be determined using IR spectroscopic methods. The IR measurements were performed on formalin-fixed, paraffin-embedded colorectal tissues from eight patients (one control, four local recurrences, three distant recurrences). A total of 128 crypts were measured. Our results showed the possibility of differentiating among control, local, and distant recurrence crypts with more than a 92% success rate using spectra measured from the crypts’ middle sites.
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Knowledge of tissue blood flow (BF) changes after free tissue transfer may enable surgeons to predict the failure of flap thrombosis at an early stage. This study used our recently developed noncontact diffuse correlation spectroscopy to monitor dynamic BF changes in free flaps without getting in contact with the targeted tissue. Eight free flaps were elevated in patients with head and neck cancer; one of the flaps failed. Multiple BF measurements probing the transferred tissue were performed during and post the surgical operation. Postoperative BF values were normalized to the intraoperative baselines (assigning “1”) for the calculation of relative BF change (rBF). The rBF changes over the seven successful flaps were 1.89±0.15, 2.26±0.13, and 2.43±0.13 (mean±standard error), respectively, on postoperative days 2, 4, and 7. These postoperative values were significantly higher than the intraoperative baseline values (p<0.001), indicating a gradual recovery of flap vascularity after the tissue transfer. By contrast, rBF changes observed from the unsuccessful flaps were 1.14 and 1.34, respectively, on postoperative days 2 and 4, indicating less flow recovery. Measurement of BF recovery after flap anastomosis holds the potential to act early to salvage ischemic flaps.
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Reconstruction of the absorption coefficient of tissue with good contrast is of key importance in functional diffuse optical imaging. A hybrid approach using model-based iterative image reconstruction and a genetic algorithm is proposed to enhance the contrast of the reconstructed image. The proposed method yields an observed contrast of 98.4%, mean square error of 0.638×10−3, and object centroid error of (0.001 to 0.22) mm. Experimental validation of the proposed method has also been provided with tissue-like phantoms which shows a significant improvement in image quality and thus establishes the potential of the method for functional diffuse optical tomography reconstruction with continuous wave setup. A case study of finger joint imaging is illustrated as well to show the prospect of the proposed method in clinical diagnosis. The method can also be applied to the concentration measurement of a region of interest in a turbid medium.
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Time-deconvolution of the instrument response from fluorescence lifetime imaging microscopy (FLIM) data is usually necessary for accurate fluorescence lifetime estimation. In many applications, however, the instrument response is not available. In such cases, a blind deconvolution approach is required. An iterative methodology is proposed to address the blind deconvolution problem departing from a dataset of FLIM measurements. A linear combination of a base conformed by Laguerre functions models the fluorescence impulse response of the sample at each spatial point in our formulation. Our blind deconvolution estimation (BDE) algorithm is formulated as a quadratic approximation problem, where the decision variables are the samples of the instrument response and the scaling coefficients of the basis functions. In the approximation cost function, there is a bilinear dependence on the decision variables. Hence, due to the nonlinear nature of the estimation process, an alternating least-squares scheme iteratively solves the approximation problem. Our proposal searches for the samples of the instrument response with a global perspective, and the scaling coefficients of the basis functions locally at each spatial point. First, the iterative methodology relies on a least-squares solution for the instrument response, and quadratic programming for the scaling coefficients applied just to a subset of the measured fluorescence decays to initially estimate the instrument response to speed up the convergence. After convergence, the final stage computes the fluorescence impulse response at all spatial points. A comprehensive validation stage considers synthetic and experimental FLIM datasets of ex vivo atherosclerotic plaques and human breast cancer cell samples that highlight the advantages of the proposed BDE algorithm under different noise and initial conditions in the iterative scheme and parameters of the proposal.
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Most glaucoma surgeries involve creating new aqueous outflow pathways with the use of a small surgical instrument. This article reported a microscope-integrated, real-time, high-speed, swept-source optical coherence tomography system (SS-OCT) with a 1310-nm light source for glaucoma surgery. A special mechanism was designed to produce an adjustable system suitable for use in surgery. A two-graphic processing unit architecture was used to speed up the data processing and real-time volumetric rendering. The position of the surgical instrument can be monitored and measured using the microscope and a grid-inserted image of the SS-OCT. Finally, experiments were simulated to assess the effectiveness of this integrated system. Experimental results show that this system is a suitable positioning tool for glaucoma surgery.
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Surgery is the most effective method to cure patients with solid tumors, and 50% of all cancer patients undergo resection. Local recurrences are due to tumor cells remaining in the wound, thus we explore near-infrared (NIR) fluorescence spectroscopy and imaging to identify residual cancer cells after surgery. Fifteen canines and two human patients with spontaneously occurring sarcomas underwent intraoperative imaging. During the operation, the wounds were interrogated with NIR fluorescence imaging and spectroscopy. NIR monitoring identified the presence or absence of residual tumor cells after surgery in 14/15 canines with a mean fluorescence signal-to-background ratio (SBR) of ∼16. Ten animals showed no residual tumor cells in the wound bed (mean SBR<2, P<0.001). None had a local recurrence at <1-year follow-up. In five animals, the mean SBR of the wound was <15, and histopathology confirmed tumor cells in the postsurgical wound in four/five canines. In the human pilot study, neither patient had residual tumor cells in the wound bed, and both remain disease free at <1.5-year follow up. Intraoperative NIR fluorescence imaging and spectroscopy identifies residual tumor cells in surgical wounds. These observations suggest that NIR imaging techniques may improve tumor resection during cancer operations.
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Microvascular network of the retina plays an important role in diagnosis and monitoring of various retinal diseases. We propose a three-dimensional (3-D) segmentation method with intensity-based Doppler variance (IBDV) based on swept-source optical coherence tomography. The automatic 3-D segmentation method is used to obtain seven surfaces of intraretinal layers. The microvascular network of the retina, which is acquired by the IBDV method, can be divided into six layers. The microvascular network of the six individual layers is visualized, and the morphology and contrast images can be improved by using the segmentation method. This method has potential for earlier diagnosis and precise monitoring in retinal vascular diseases.
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Automatic identification of fungi in microscopic fecal images provides important information for evaluating digestive diseases. To date, disease diagnosis is primarily performed by manual techniques. However, the accuracy of this approach depends on the operator’s expertise and subjective factors. The proposed system automatically identifies fungi in microscopic fecal images that contain other cells and impurities under complex environments. We segment images twice to obtain the correct area of interest, and select ten features, including the circle number, concavity point, and other basic features, to filter fungi. An artificial neural network (ANN) system is used to identify the fungi. The first stage (ANN-1) processes features from five images in differing focal lengths; the second stage (ANN-2) identifies the fungi using the ANN-1 output values. Images in differing focal lengths can be used to improve the identification result. The system output accurately detects the image, whether or not it has fungi. If the image does have fungi, the system output counts the number of different fungi types.
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In dental health care, the application of ultrashort laser pulses enables dental tissue ablation free from thermal side effects, such as melting and cracking. However, these laser types create undesired micro- and nanoparticles, which might cause a health risk for the patient or surgeon. The aim of this study was to investigate the driving mechanisms of micro- and nanoparticle formation during ultrashort pulse laser ablation of dental tissue. Time-resolved microscopy was chosen to observe the ablation dynamics of mammoth ivory after irradiation with 660 fs laser pulses. The results suggest that nanoparticles might arise in the excited region. The thermal expansion of the excited material induces high pressure in the surrounding bulk tissue, generating a pressure wave. The rarefaction wave behind this pressure wave causes spallation, leading to ejection of microparticles.
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A simple, affordable method for imaging through biological tissue is investigated. The method consists of (1) imaging with a wavelength that has a relatively small scattering coefficient (1310 nm in this case) and (2) collimated illumination together with (3) focal plane detection to enhance the detection of the ballistic photons relative to the diffusive light. We demonstrate ballistic detection of an object immersed in a 1-cm-thick cuvette filled with 4% Intralipid, which is equivalent to ∼1 to 2 cm of skin tissue. With the same technology, a ballistic image of a 1-mm-wide object in 10-mm-thick chicken breast is also presented.
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We have developed a compact dual-view endoscopic probe without field obscuration to address the need of simultaneously observing forward and backward fields of view (FOVs) in the colon. The objective is compact with the forward-view and rear-view optical paths sharing the same optical elements. The compact objective is new in that no FOV is blocked. The illumination for forward-view imaging is provided by the cylindrical light guide and backward illumination is achieved with a reflector. We have designed, prototyped, and tested the endoscope by comparing it to a standard clinical colonoscope. We will discuss the system concept, objective design, fabrication of the freeform lens, and test results.
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In a previous report, we demonstrated master-slave optical coherence tomography (MS-OCT), an OCT method that does not need resampling of data and can be used to deliver en face images from several depths simultaneously. In a separate report, we have also demonstrated MS-OCT’s capability of producing cross-sectional images of a quality similar to those provided by the traditional Fourier domain (FD) OCT technique, but at a much slower rate. Here, we demonstrate that by taking advantage of the parallel processing capabilities offered by the MS-OCT method, cross-sectional OCT images of the human retina can be produced in real time. We analyze the conditions that ensure a true real-time B-scan imaging operation and demonstrate in vivo real-time images from human fovea and the optic nerve, with resolution and sensitivity comparable to those produced using the traditional FD-based method, however, without the need of data resampling.
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We describe the use of combined ultrasonic imaging (USI) and photoacoustic radar imaging (PARI) with linear chirp laser modulation to provide visualization of blood with and without the use of gold nanoparticles. A blood vessel simulating sample (S1) containing pure sheep blood was shown to be an optically weak absorbing medium which satisfies thermal but not acoustic confinement. On the contrary, the blood-gold combinations (S2) using 10% and S3 (20%) Au concentrations behaved as optically strongly absorbing media. A heating efficiency of 0.54 to 8.60×103 K cm2 J−1 was determined for Au NPs. The optimal optical power modulation spectral density was determined to be in the range of 0.5 to 0.8 MHz and 0.3 to 1.0 MHz for USI and PARI, respectively. USI produced a better structural image while PARI produced a better functional image of the simulated blood vessel in the order of S2 > S3 > S1 due to enhanced signal-to-noise ratio. Two-dimensional images of the simulated blood vessel were also obtained. In summary, the PA signal does not increase linearly with Au NP concentration and the change of blood osmolarity due to temperature increase can cause thermo-hemolysis of red blood cells which in turn degrades the PA signal and thus the blood imaging quality. On the other hand, USI produced the best structural image, S4, due to the strong US reflection response from Au NPs and its insensitivity to the presence of blood.
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Diffuse reflectance spectroscopy (DRS) has been extensively used for characterization of biological tissues as a noninvasive optical technique to evaluate the optical properties of tissue. We investigated a method for evaluating the reduced scattering coefficient μs′, the absorption coefficient μa, the tissue oxygen saturation StO2, and the reduction of heme aa3 in cytochrome c oxidase CcO of in vivo liver tissue using a single-reflectance fiber probe with two source-collector geometries. We performed in vivo recordings of diffuse reflectance spectra for exposed rat liver during the ischemia–reperfusion induced by the hepatic portal (hepatic artery, portal vein, and bile duct) occlusion. The time courses of μa at 500, 530, 570, and 584 nm indicated the hemodynamic change in liver tissue as well as StO2. Significant increase in μa(605)/μa(620) during ischemia and after euthanasia induced by nitrogen breathing was observed, which indicates the reduction of heme aa3, representing a sign of mitochondrial energy failure. The time courses ofμs′ at 500, 530, 570, and 584 nm were well correlated with those of μa, which also reflect the scattering by red blood cells. On the other hand, at 700 and 800 nm, a temporary increase in μs′ and an irreversible decrease in μs′ were observed during ischemia–reperfusion and after euthanasia induced by nitrogen breathing, respectively. The change in μs′ in the near-infrared wavelength region during ischemia is indicative of the morphological changes in the cellular and subcellular structures induced by the ischemia, whereas that after euthanasia implies the hepatocyte vacuolation. The results of the present study indicate the potential application of the current DRS system for evaluating the pathophysiological conditions of in vivo liver tissue.
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Standard clinical management of extremity soft tissue sarcomas includes surgery with radiation therapy. Wound complications (WCs) arising from treatment may occur due to bacterial infection and tissue breakdown. The ability to detect changes in these parameters during treatment may lead to earlier interventions that mitigate WCs. We describe the use of a new system composed of an autofluorescence imaging device and an optical three-dimensional tracking system to detect and coregister the presence of bacteria with radiation doses. The imaging device visualized erythema using white light and detected bacterial autofluorescence using 405-nm excitation light. Its position was tracked relative to the patient using IR reflective spheres and registration to the computed tomography coordinates. Image coregistration software was developed to spatially overlay radiation treatment plans and dose distributions on the white light and autofluorescence images of the surgical site. We describe the technology, its use in the operating room, and standard operating procedures, as well as demonstrate technical feasibility and safety intraoperatively. This new clinical tool may help identify patients at greater risk of developing WCs and investigate correlations between radiation dose, skin response, and changes in bacterial load as biomarkers associated with WCs.
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We propose an offset-sparsity decomposition method for the enhancement of a color microscopic image of a stained specimen. The method decomposes vectorized spectral images into offset terms and sparse terms. A sparse term represents an enhanced image, and an offset term represents a “shadow.” The related optimization problem is solved by computational improvement of the accelerated proximal gradient method used initially to solve the related rank-sparsity decomposition problem. Removal of an image-adapted color offset yields an enhanced image with improved colorimetric differences among the histological structures. This is verified by a no-reference colorfulness measure estimated from 35 specimens of the human liver, 1 specimen of the mouse liver stained with hematoxylin and eosin, 6 specimens of the mouse liver stained with Sudan III, and 3 specimens of the human liver stained with the anti-CD34 monoclonal antibody. The colorimetric difference improves on average by 43.86% with a 99% confidence interval (CI) of [35.35%, 51.62%]. Furthermore, according to the mean opinion score, estimated on the basis of the evaluations of five pathologists, images enhanced by the proposed method exhibit an average quality improvement of 16.60% with a 99% CI of [10.46%, 22.73%].
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Regenerative medicine brings promising applications for mesenchymal stem cells, such as dental pulp stem cells (DPSCs). Confocal Raman microscopy, a noninvasive technique, is used to study osteogenic differentiation of DPSCs. Integrated Raman intensities in the 2800 to 3000 cm−1 region (C-H stretching) and the 960 cm−1 peak (ν1PO43−) were collected (to image cells and phosphate, respectively), and the ratio of two peaks 1660 over 1690 cm−1 (amide I bands) to measure the collagen cross-linking has been calculated. Raman spectra of DPSCs after 21 days differentiation reveal several phosphate peaks: ν1 (first stretching mode) at 960 cm−1,ν2at 430 cm−1, and ν4 at 585 cm−1 and collagen cross-linking can also be calculated. Confocal Raman microscopy enables monitoring osteogenic differentiation in vitro and can be a credible tool for clinical stem cell based research.
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We have developed ultraviolet (UV)- and visible-light one-shot spectral domain (SD) optical coherence tomography (OCT) that enables in situ imaging of human skin with an arbitrary wavelength in the UV–visible-light region (370–800 nm). We alleviated the computational burden for each color OCT image by physically dispersing the irradiating light with a color filter. The system consists of SD-OCT with multicylindrical lenses; thus, mechanical scanning of the mirror or stage is unnecessary to obtain an OCT image. Therefore, only a few dozens of milliseconds are necessary to obtain single-image data. We acquired OCT images of one subject’s skin in vivo and of a skin excision ex vivo for red (R, 650±20 nm), green (G, 550±20 nm), blue (B, 450±20 nm), and UV (397±5 nm) light. In the visible-light spectrum, R light penetrated the skin and was reflected at a lower depth than G or B light. On the skin excision, we demonstrated that UV light reached the dermal layer. We anticipated that basic knowledge about the spectral properties of human skin in the depth direction could be acquired with this system.
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Detection of an early stage of subglottic edema is vital for airway management and prevention of stenosis, a life-threatening condition in critically ill neonates. As an observer for the task of diagnosing edema in vivo, we investigated spatiotemporal correlation (STC) of full-range optical coherence tomography (OCT) images acquired in the rabbit airway with experimentally simulated edema. Operating the STC observer on OCT images generates STC coefficients as test statistics for the statistical decision task. Resulting from this, the receiver operating characteristic (ROC) curves for the diagnosis of airway edema with full-range OCT in-vivo images were extracted and areas under ROC curves were calculated. These statistically quantified results demonstrated the potential clinical feasibility of the STC method as a means to identify early airway edema.
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We used ultrasound (US) and photoacoustic (PA) imaging modalities to characterize cattle trabecular bones. The PA signals were generated with an 805-nm continuous wave laser used for optimally deep optical penetration depth. The detector for both modalities was a 2.25-MHz US transducer with a lateral resolution of ∼1 mm at its focal point. Using a lateral pixel size much larger than the size of the trabeculae, raster scanning generated PA images related to the averaged values of the optical and thermoelastic properties, as well as density measurements in the focal volume. US backscatter yielded images related to mechanical properties and density in the focal volume. The depth of interest was selected by time-gating the signals for both modalities. The raster scanned PA and US images were compared with microcomputed tomography (μCT) images averaged over the same volume to generate similar spatial resolution as US and PA. The comparison revealed correlations between PA and US modalities with the mineral volume fraction of the bone tissue. Various features and properties of these modalities such as detectable depth, resolution, and sensitivity are discussed.
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We synthesized and characterized gold nanostars and their silica-coated derivatives with 7- to 50-nm shell thicknesses as contrast agents for optical imaging. The scattering and absorption coefficients of the nanoparticles (NPs) were estimated by means of collimated transmittance and diffuse reflectance/transmittance analyses. The contrasting properties of the nanostructures were studied in optical coherence tomography glass capillary imaging. The silica-coated nanostars with the thickest shell have higher scattering ability in comparison with bare nanostars. Viability assays confirmed weak in vitro toxicity of nanostructures at up to ∼200-μg/mL concentrations. We showed real-time visualization of nanostars in both agarose and cultured cells by analyzing the backscattering signal using a conventional laser confocal microscope. The signal intensity detected from the silica-coated NPs was almost 1.5 times higher in comparison with bare nanostars. To the best of our knowledge, this is the first time that conventional laser confocal microscopy was applied in combined scattering and transmitted light modes to detect the backscattered signal of gold nanostars, which is useful for direct monitoring of the uptake, translocation, and accumulation of NPs in living cells.
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The objective is to characterize the impact of different ablation parameters on the thermal load during corneal refractive surgery by means of excimer laser ablation on porcine eyes. One hundred eleven ablations were performed in 105 porcine eyes. Each ablation was recorded using infrared thermography and analyzed mainly based on the two tested local frequencies (40 Hz, clinical local frequency; 1000 Hz, no local frequency). The change in peak corneal temperature was analyzed with respect to varying ablation parameters [local frequency, system repetition rate, pulse energy, optical zone (OZ) size, and refractive correction]. Transepithelial ablations were also compared to intrastromal ablations. The average of the baseline temperature across all eyes was 20.5°C±1.1 (17.7°C to 22.2°C). Average of the change in peak corneal temperature for all clinical local frequency ablations was 5.8°C±0.8 (p=3.3E−53 to baseline), whereas the average was 9.0°C±1.5 for all no local frequency ablations (p=1.8E−35 to baseline, 1.6E−16 to clinical local frequency ablations). A logarithmic relationship was observed between the changes in peak corneal temperature with increasing local frequency. For clinical local frequency, change in peak corneal temperature was comparatively flat (r2=0.68 with a range of 1.5°C) with increasing system repetition rate and increased linearly with increasing OZ size (r2=0.95 with a range of 2.4°C). Local frequency controls help maintain safe corneal temperature increase during excimer laser ablations. Transepithelial ablations induce higher thermal load compared to intrastromal ablations, indicating a need for stronger thermal controls in transepithelial refractive procedures.
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Laser photobiomodulation can improve bone healing, but well-defined treatment parameters are lacking. Saos-2 human osteoblast-like cells were subjected to an in vitro scratch-wound healing assay and irradiated by a 915-nm gallium-aluminum-arsenide diode laser for 0, 48, 96, and 144 s using doses of, respectively, 0, 5, 10, and 15 J/cm2. Wound area was measured after 4, 24, 48, and 72 h. Cell viability, DNA content, gene expression, and release of bone-related proteins were evaluated after 24, 48, and 72 h. Laser significantly improved wound healing compared with nonirradiated controls. Cells treated with laser doses of 5 and 10 J/cm2reached wound closure after 72 h, followed by 15 J/cm2after 96 h. With the cell proliferation inhibitor Mitomycin C, the doses of 10 and 15 J/cm2maintained an improved wound healing compared with controls. Laser increased collagen type 1 gene expression with higher doses inducing a longer-lasting effect, whereas transforming growth factor-beta 1 showed comparable or decreased levels in irradiated versus nonirradiated groups, with no effect on protein release. This study demonstrated that laser photobiomodulation at 915 nm promoted wound healing mainly through stimulation of cell migration and collagen deposition by osteoblasts.
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The uptake of E-combretastatins, potential prodrugs of the anticancer Z-isomers, into multicellular spheroids has been imaged by intrinsic fluorescence in three dimensions using two-photon excited fluorescence lifetime imaging with 625-nm ultrafast femtosecond laser pulses. Uptake is initially observed at the spheroid periphery but extends to the spheroid core within 30 min. Using agarose gels as a three-dimensional model, the conversion of Z(trans)→E(cis) via two-photon photoisomerization is demonstrated and the location of this photochemical process may be precisely selected within the micron scale in all three dimensions at depths up to almost 2 mm. We discuss these results for enhanced tissue penetration at longer near-infrared wavelengths for cancer therapy and up to three-photon excitation and imaging using 930-nm laser pulses with suitable combretastatin analogs.
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