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22 October 2015 Effect of low-level laser therapy on irradiated parotid glands—study in mice
Monique Dossena Acauan, Ana Paula Gomes, Aroldo Braga-Filho, Maria Antonia Zancanaro de Figueiredo, Karen Cherubini, Fernanda G. Salum
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Journal of Biomedical Optics, Vol. 20, Issue 10, 108002 (October 2015). https://doi.org/10.1117/1.JBO.20.10.108002
Abstract
The objective of this study was to evaluate the effect of low-level laser therapy (LLLT) on radiotherapy-induced morphological changes and caspase-3 immunodetection in parotids of mice. Forty-one Swiss mice were divided into control, radiotherapy, 2- and 4-J laser groups. The experimental groups were exposed to ionizing radiation in a single session of 10 Gy. In the laser groups, a GaAlAs laser (830 nm, 100 mW, 0.028  cm2, 3.57  W/cm2) was used on the region corresponding to the parotid glands, with 2-J energy (20 s, 71  J/cm2) or 4 J (40 s, 135  J/cm2) per point. LLLT was performed immediately before and 24 h after radiotherapy. One point was applied in each parotid gland. The animals were euthanized 48 h or 7 days after radiotherapy and parotid glands were dissected for morphological analysis and immunodetection of caspase-3. There was no significant difference between groups in the immunodetection of caspase-3, but the laser groups had a lower percentage compared to the radiotherapy group. LLLT promoted the preservation of acinar structure, reduced the occurrence of vacuolation, and stimulated parotid gland vascularization. Of the two LLLT protocols, the one using 4 J of energy showed better results.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2015/$25.00 © 2015 SPIE
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Monique Dossena Acauan, Ana Paula Gomes, Aroldo Braga-Filho, Maria Antonia Zancanaro de Figueiredo, Karen Cherubini, and Fernanda G. Salum "Effect of low-level laser therapy on irradiated parotid glands—study in mice," Journal of Biomedical Optics 20(10), 108002 (22 October 2015). https://doi.org/10.1117/1.JBO.20.10.108002
Published: 22 October 2015
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KEYWORDS
Radiotherapy
Laser therapeutics
Cell death
Proteins
Ionizing radiation
Calibration
Head
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