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2 July 2015 Investigation of biochemical property changes in activation-induced CD8+ T cell apoptosis using Raman spectroscopy
Young Ju Lee, Hyung Joon Ahn, Gi-Ja Lee, Gyeong Bok Jung, Gihyun Lee, Dohyun Kim, Jae-Ho Shin, Kyung-Hyun Jin, Hun-Kuk Park
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Journal of Biomedical Optics, Vol. 20, Issue 7, 075001 (July 2015). https://doi.org/10.1117/1.JBO.20.7.075001
Abstract
The study was to investigate the changes in biochemical properties of activated mature CD8+ T cells related to apoptosis at a molecular level. We confirmed the activation and apoptosis of CD8+ T cells by fluorescence-activated cell sorting and atomic force microscopy and then performed Raman spectral measurements on activated mature CD8+ T cells and cellular deoxyribose nucleic acid (DNA). In the activated mature CD8+ T cells, there were increases in protein spectra at 1002 and 1234  cm−1. In particular, to assess the apoptosis-related DNA spectral signatures, we investigated the spectra of the cellular DNA isolated from resting and activated mature CD8+ T cells. Raman spectra at 765 to 786  cm−1 and 1053 to 1087  cm−1 were decreased in activated mature DNA. In addition, we analyzed Raman spectrum using the multivariate statistical method including principal component analysis. Raman spectra of activated mature DNA are especially well-discriminated from those of resting DNA. Our findings regarding the biochemical and structural changes associated with apoptosis in activated mature T cells and cellular DNA according to Raman spectroscopy provide important insights into allospecific immune responses generated after organ transplantation, and may be useful for therapeutic manipulation of the immune response.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Young Ju Lee, Hyung Joon Ahn, Gi-Ja Lee, Gyeong Bok Jung, Gihyun Lee, Dohyun Kim, Jae-Ho Shin, Kyung-Hyun Jin, and Hun-Kuk Park "Investigation of biochemical property changes in activation-induced CD8+ T cell apoptosis using Raman spectroscopy," Journal of Biomedical Optics 20(7), 075001 (2 July 2015). https://doi.org/10.1117/1.JBO.20.7.075001
Published: 2 July 2015
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Cited by 9 scholarly publications.
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KEYWORDS
Raman spectroscopy
Cell death
Proteins
Principal component analysis
Atomic force microscopy
Statistical analysis
Flow cytometry
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