Vulnerable atherosclerotic plaque detection by resonance Raman spectroscopy

Cheng-hui Liu; Susie Boydston-White; Arel Weisberg; Wubao Wang; Laura A. Sordillo; Adler Perotte; Vincent P. Tomaselli; Peter P. Sordillo; Zhe Pei; Lingyan Shi; Robert R. Alfano

doi:10.1117/1.JBO.21.12.127006

21 December 2016 Vulnerable atherosclerotic plaque detection by resonance Raman spectroscopy

Cheng-hui Liu, Susie Boydston-White, Arel Weisberg, Wubao Wang, Laura A. Sordillo, Adler Perotte, Vincent P. Tomaselli, Peter P. Sordillo, Zhe Pei, Lingyan Shi, Robert R. Alfano

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Journal of Biomedical Optics, Vol. 21, Issue 12, 127006 (December 2016). https://doi.org/10.1117/1.JBO.21.12.127006

Abstract

A clear correlation has been observed between the resonance Raman (RR) spectra of plaques in the aortic tunica intimal wall of a human corpse and three states of plaque evolution: fibrolipid plaques, calcified and ossified plaques, and vulnerable atherosclerotic plaques (VPs). These three states of atherosclerotic plaque lesions demonstrated unique RR molecular fingerprints from key molecules, rendering their spectra unique with respect to one another. The vibrational modes of lipids, cholesterol, carotenoids, tryptophan and heme proteins, the amide I, II, III bands, and methyl/methylene groups from the intrinsic atherosclerotic VPs in tissues were studied. The salient outcome of the investigation was demonstrating the correlation between RR measurements of VPs and the thickness measurements of fibrous caps on VPs using standard histopathology methods, an important metric in evaluating the stability of a VP. The RR results show that VPs undergo a structural change when their caps thin to 66 μm, very close to the 65-μm empirical medical definition of a thin cap fibroatheroma plaque, the most unstable type of VP.

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Cheng-hui Liu, Susie Boydston-White, Arel Weisberg, Wubao Wang, Laura A. Sordillo, Adler Perotte, Vincent P. Tomaselli, Peter P. Sordillo, Zhe Pei, Lingyan Shi, and Robert R. Alfano "Vulnerable atherosclerotic plaque detection by resonance Raman spectroscopy," Journal of Biomedical Optics 21(12), 127006 (21 December 2016). https://doi.org/10.1117/1.JBO.21.12.127006

Received: 7 October 2016; Accepted: 28 November 2016; Published: 21 December 2016

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