In vivo/ex vivo targeting of Langerhans cells after topical application of the immune response modifier TMX-202: confocal Raman microscopy and histology analysis

Maxim E. Darvin; Gisela Thiede; Saul Mujica Ascencio; Sabine Schanzer; Heike Richter; Sabrina E. Vinzón; Daniel Hasche; Frank Rösl; Roberto May; Yohan Hazot; Dov Tamarkin; Juergen Lademann

doi:10.1117/1.JBO.21.5.055004

27 May 2016 In vivo/ex vivo targeting of Langerhans cells after topical application of the immune response modifier TMX-202: confocal Raman microscopy and histology analysis

Maxim E. Darvin, Gisela Thiede, Saul Mujica Ascencio, Sabine Schanzer, Heike Richter, Sabrina E. Vinzón, Daniel Hasche, Frank Rösl, Roberto May, Yohan Hazot, Dov Tamarkin, Juergen Lademann

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Journal of Biomedical Optics, Vol. 21, Issue 5, 055004 (May 2016). https://doi.org/10.1117/1.JBO.21.5.055004

Abstract

The increased ability of TMX-202 (derivative of imiquimod) to penetrate the intact stratum corneum (SC) and the follicular orifices of porcine ear skin was shown ex vivo using confocal Raman microscopy and laser scanning microscopy. Moreover, to assess whether TMX-202 is able to reach the immune cells, Langerhans cells extracted from pretreated human skin were investigated ex vivo using confocal Raman microscopy combined with multivariate statistical methods. Tracking the Raman peak of dimethyl sulfoxide centered at 690 cm⁻¹, the absorption of TMX-202 containing formulation by Langerhans cells was shown. To answer the question whether the TMX-202 active ingredient is able to reach Langerhans cells, the attraction of immune cells to TMX-202 containing formulation treated skin was measured in the in vivo rodent model Mastomys coucha. The results show that TMX-202 active ingredient is able to reach Langerhans cells after penetrating through the intact skin and subsequently attract immune cells. Both the intercellular/transcellular as well as the follicular pathways allow the penetration through the intact barrier of the SC.

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Maxim E. Darvin, Gisela Thiede, Saul Mujica Ascencio, Sabine Schanzer, Heike Richter, Sabrina E. Vinzón, Daniel Hasche, Frank Rösl, Roberto May, Yohan Hazot, Dov Tamarkin, and Juergen Lademann "In vivo/ex vivo targeting of Langerhans cells after topical application of the immune response modifier TMX-202: confocal Raman microscopy and histology analysis," Journal of Biomedical Optics 21(5), 055004 (27 May 2016). https://doi.org/10.1117/1.JBO.21.5.055004

Published: 27 May 2016

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