ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems

Veronica Sorgato; Michel Berger; Charlotte Emain; Christine Vever-Bizet; Jean-Marc Dinten; Geneviève Bourg-Heckly; Anne Planat-Chrétien

doi:10.1117/1.JBO.21.6.065003

9 June 2016 ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems

Veronica Sorgato, Michel Berger, Charlotte Emain, Christine Vever-Bizet, Jean-Marc Dinten, Geneviève Bourg-Heckly, Anne Planat-Chrétien

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Journal of Biomedical Optics, Vol. 21, Issue 6, 065003 (June 2016). https://doi.org/10.1117/1.JBO.21.6.065003

Abstract

We have developed an adaptive calibration algorithm and protocol (ACA-Pro) that corrects from the instrumental response of various spatially resolved diffuse reflectance spectroscopy (DRSsr) systems to enable the quantification of absorption and scattering properties based on a Monte Carlo-based look-up-table approach. The protocol involves the use of a calibration reference base built with measurements of a range of different diffusive intralipid phantoms. Moreover, an advanced strategy was established to take into account the experimental variations with an additional measurement of a common solid material, allowing the use of a single calibration reference base for all experiments. The ACA-Pro is validated in contact and noncontact probe-based DRSsr systems. Furthermore, the first results of a setup replacing the probe with a CCD detector are shown to confirm the robustness of the approach.

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Veronica Sorgato, Michel Berger, Charlotte Emain, Christine Vever-Bizet, Jean-Marc Dinten, Geneviève Bourg-Heckly, and Anne Planat-Chrétien "ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems," Journal of Biomedical Optics 21(6), 065003 (9 June 2016). https://doi.org/10.1117/1.JBO.21.6.065003

Published: 9 June 2016

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