CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 22 > Issue 4 > Article
Translator Disclaimer
Open Access
10 April 2017 Listening to membrane potential: photoacoustic voltage-sensitive dye recording
Haichong K. Zhang, Ping Yan, Jeeun Kang, Diane S. Abou, Hanh N. D. Le, Abhinav K. Jha, Daniel L. J. Thorek, Jin U. Kang, Arman Rahmim, Dean F. Wong, Emad M. Boctor, Leslie M. Loew
Author Affiliations +
J. of Biomedical Optics, 22(4), 045006 (2017). https://doi.org/10.1117/1.JBO.22.4.045006
Abstract
Voltage-sensitive dyes (VSDs) are designed to monitor membrane potential by detecting fluorescence changes in response to neuronal or muscle electrical activity. However, fluorescence imaging is limited by depth of penetration and high scattering losses, which leads to low sensitivity in vivo systems for external detection. By contrast, photoacoustic (PA) imaging, an emerging modality, is capable of deep tissue, noninvasive imaging by combining near-infrared light excitation and ultrasound detection. Here, we show that voltage-dependent quenching of dye fluorescence leads to a reciprocal enhancement of PA intensity. We synthesized a near-infrared photoacoustic VSD (PA-VSD), whose PA intensity change is sensitive to membrane potential. In the polarized state, this cyanine-based probe enhances PA intensity while decreasing fluorescence output in a lipid vesicle membrane model. A theoretical model accounts for how the experimental PA intensity change depends on fluorescence and absorbance properties of the dye. These results not only demonstrate PA voltage sensing but also emphasize the interplay of both fluorescence and absorbance properties in the design of optimized PA probes. Together, our results demonstrate PA sensing as a potential new modality for recording and external imaging of electrophysiological and neurochemical events in the brain.
Citation Download Citation
Haichong K. Zhang, Ping Yan, Jeeun Kang, Diane S. Abou, Hanh N. D. Le, Abhinav K. Jha, Daniel L. J. Thorek, Jin U. Kang, Arman Rahmim, Dean F. Wong, Emad M. Boctor, and Leslie M. Loew "Listening to membrane potential: photoacoustic voltage-sensitive dye recording," Journal of Biomedical Optics 22(4), 045006 (10 April 2017). https://doi.org/10.1117/1.JBO.22.4.045006
Received: 1 December 2016; Accepted: 17 March 2017; Published: 10 April 2017
JOURNAL ARTICLE
 9 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
SHARE
GET CITATION
Lens.org Logo
CITATIONS
Cited by 32 scholarly publications.
Advertisement
Advertisement
KEYWORDS
Luminescence
Absorbance
Photoacoustic spectroscopy
Voltage sensitive dyes
Brain
Ultrasonography
Neuroimaging
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top