Label-free real-time optical monitoring of DNA hybridization using SiN Mach–Zehnder interferometer-based integrated biosensing platform

Mohammed Sharif Murib; Daan Martens; Peter Bienstman

doi:10.1117/1.JBO.23.12.127002

21 December 2018 Label-free real-time optical monitoring of DNA hybridization using SiN Mach–Zehnder interferometer-based integrated biosensing platform

Mohammed Sharif Murib, Daan Martens, Peter Bienstman

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Journal of Biomedical Optics, Vol. 23, Issue 12, 127002 (December 2018). https://doi.org/10.1117/1.JBO.23.12.127002

Abstract

We report on the label-free real-time optical monitoring of DNA hybridization upon exposure to a flow of complementary DNA at different concentrations. The biosensor is composed of a silicon nitride integrated unbalanced Mach–Zehnder interferometer (MZI), with an integrated arrayed waveguide grating as a spectral filter. This MZI has been shown to have both sufficient multiplexing capability and limit of detection on the order of ^{10 − 6} RIU. Probe DNA, consisting of a 36-mer fragment is covalently immobilized on the silicon nitride integrated biosensor. The wavelength shift is monitored upon complementary DNA targets being flown over the sensor. Concentrations of 1 pM can be easily detected. Also, an alternative route to modify the sensor surface with carboxylic groups using the photochemical reaction of fatty acids is proposed and preliminary XPS results are presented. Moreover, preliminary results for DNA obtained from a rolling circle amplification (RCA-DNA) process and spiked in a realistic amplification buffer are presented.

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Mohammed Sharif Murib, Daan Martens, and Peter Bienstman "Label-free real-time optical monitoring of DNA hybridization using SiN Mach–Zehnder interferometer-based integrated biosensing platform," Journal of Biomedical Optics 23(12), 127002 (21 December 2018). https://doi.org/10.1117/1.JBO.23.12.127002

Received: 15 May 2018; Accepted: 3 December 2018; Published: 21 December 2018

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