Widefield and total internal reflection fluorescent structured illumination microscopy with scanning galvo mirrors

Youhua Chen; Ruizhi Cao; Wenjie Liu; Dazhao Zhu; Zhiming Zhang; Cuifang Kuang; Xu Liu

doi:10.1117/1.JBO.23.4.046007

24 April 2018 Widefield and total internal reflection fluorescent structured illumination microscopy with scanning galvo mirrors

Youhua Chen, Ruizhi Cao, Wenjie Liu, Dazhao Zhu, Zhiming Zhang, Cuifang Kuang, Xu Liu

Author Affiliations +

Journal of Biomedical Optics, Vol. 23, Issue 4, 046007 (April 2018). https://doi.org/10.1117/1.JBO.23.4.046007

Abstract

We present an alternative approach to realize structured illumination microscopy (SIM), which is capable for live cell imaging. The prototype utilizes two sets of scanning galvo mirrors, a polarization converter and a piezo-platform to generate a fast shifted, s-polarization interfered and periodic variable illumination patterns. By changing the angle of the scanning galvanometer, we can change the position of the spots at the pupil plane of the objective lens arbitrarily, making it easy to switch between widefield and total internal reflection fluorescent-SIM mode and adapting the penetration depth in the sample. Also, a twofold resolution improvement is achieved in our experiments. The prototype offers more flexibility of pattern period and illumination orientation changing than previous systems.

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Youhua Chen, Ruizhi Cao, Wenjie Liu, Dazhao Zhu, Zhiming Zhang, Cuifang Kuang, and Xu Liu "Widefield and total internal reflection fluorescent structured illumination microscopy with scanning galvo mirrors," Journal of Biomedical Optics 23(4), 046007 (24 April 2018). https://doi.org/10.1117/1.JBO.23.4.046007

Received: 17 January 2018; Accepted: 3 April 2018; Published: 24 April 2018

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KEYWORDS

Mirrors

Polarization

Modulation

Imaging systems

Microscopy

Luminescence

Objectives

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