Peculiarities of control of erythrocytes moving in an evanescent field

Abstract. An investigation of the influence of an evanescent wave on the dynamics of motion of erythrocytes in blood plasma is presented. Computer simulation of erythrocytes moving in an evanescent field and experimental demonstration of the forecasted motion substantiate the possibility for control of position of red blood cells in a solution. The range of velocities of transversal motion of erythrocytes due to the action of the optical force of the generated evanescent field is determined as a function of the angle of illumination of a cell by a linearly polarized wave with the azimuth of polarization 45 deg.


Introduction
A majority of investigations in biology and medicine carried out over the last decades are devoted to development of methods for studying biological objects with visualization of micromanipulations at molecular and cellular scales. Noninvasive optical trapping and moving the microobjects presume application of approaches characterized by superhigh accuracy 1 used for investigations of biomarkers, 2 DNA molecules, 3,4 myosin, 5 kinesin, 6 etc. As a rule, controlled manipulations of microobjects are performed using optical tweezers. (This approach and term have been proposed by Ashkin in 1970. 7 ) At present, a set of techniques is available for manipulation of biological objects and microparticles using optical and magnetic tweezers, as well as atom-force microscopy and raysounding of cells. Specifically, the optical techniques provide noninvasive, contactless trapping of microparticles or organelles in cells, by transfer of the linear momentum from a photon. Optical tweezers are used as the primary tool in biophysical investigations 8 for measuring the dissolving, studying kinetochore-microtubule interaction 9 and quantifying intercellular interactions. 10 Traditionally, an optical trap is a sharply focused laser beam formed by a microscope objective with a high numerical aperture, which enables control of position and motion of microobjects by applying optical force. The magnitude of the optical force is on the order of a few pN, and the physical mechanism of the action of this force creates the interaction of EM radiation with the induced dipole momentum of the dielectric particles. Transfer of the linear momentum from light to particles depends on the optical properties of the controlled microparticles and on the phase distribution and polarization of the probing beam.
Taking into account optical diffraction, the study of smallsized systems is delicate due to limitation on the maximal magnitude of the energy density achieved in optical tweezers for a fixed radiation power. This limitation is of importance for biological samples, which can be destroyed by high-power radiation.
Widespread plasmonic optical tweezers, 11 so-called plasmonic nanotubes with metallic nanoantennas, are used for amplification and concentration of energy of optical fields within deep subwavelength gaps. This provides high magnitudes of the intensity gradient in the near field and results in growing optical forces for a fixed input radiation power, being lower than the threshold for biomaterial damage by two to three orders of magnitude.
Therefore, it is of importance to search for techniques for manipulation of biological microobjects at the cell scale in vivo, by the use of physiological permeability of biomembranes. The use of optical tweezers for trapping and manipulating erythrocytes in blood capillaries makes it possible to clean microvessels and renew their permeability owing to combination of the results of biological investigations in vitro and in vivo. 12 The ability of laser radiation to penetrate biological tissues enables creation of systems for noninvasive manipulation of biological micro-and nano-objects in living organisms, including realization of controlled spatial motion of such objects, while avoiding tissue destruction.
Here, we investigate the influence of laser radiation on erythrocytes (red blood cells, RBCs), both for optical trapping and moving, and for realization of precise control of such objects at the nanoscale level, providing desirable velocity of motion in the evanescent field due to changing polarization of a laser beam and change in illumination conditions. This approach enables control of blood vessels using low-power laser systems and gradual renewal of blood supplying necrotic tissues. Evanescent waves excited above the interface of two media with different refraction indices are the complementary source of optical forces that can be employed for manipulation of microparticles in a broader range of applications than conventional tweezers. Note, special attention is paid to waveguide structures, optical fibers, and optical fiber-based structures such as trapper, subwavelength wire, and fiber-ring, providing control and capturing of isolated molecules, microspheres, 13 and massive dielectric particles. 14,15 Investigations in 2011 15 have demonstrated the possibility of using a defect-decorated optical nanofiber for targeted delivery of nanoparticles. Progress of diagnostic and therapeutic techniques used in practical medicine promotes further search for tools for controlled movement of nano-objects, including RBCs in biological systems.
Developing the approaches proposed in the given paper, one can either look for the conditions for the realization of an evanescent wave at the interface of the derma-blood vessel (RBS), thereby providing noninvasive methods for controlling the erythrocyte motion, or taking invasive intervention with a pointed rectangular fiber and correctly selected refraction index. This facilitates the realization of vertical spin of the evanescent wave for erythrocyte manipulation.
Thus, the purpose of this work is to study the influence of the optical evanescent wave on erythrocytes, including experimental demonstration of controlled location and motion of a cell, depending on the illumination conditions.

Simulation of Interaction of Erythrocytes with an Evanescent Wave
Intense investigations of the optical forces acting on micro-and nanoparticles localized near interface of two media are carried out here for elucidating the distribution of the components of an optical field above a microstructured surface. Complex geometry of a surface and surface inhomogeneities may considerably reinforce the optical forces and cause appearance of new components connected, in part, with manifestation of the extraordinary vertical spin angular momentum of an evanescent wave. 16 Miniaturization of the optical traps leads to creation of controlled microspheres, which act as micropumps and switches. From this standpoint, the paper 17 is of special interest devoted to the study of the behavior of microparticles located at the distances of a few micrometers from a surface, viz. at the evanescent field where one must take into account both optical and hydrodynamic surface effects. Taken together, this provides the description of complex motion of the particles captured by an evanescent wave in two-dimensional representation, with formation the trace as an elliptic form at the plane of extinction of an evanescent wave. Meanwhile, in the case of isolated erythrocytes, the question on feasibilities for controlled motion of these cells using an evanescent wave excited at the boundary "prism-blood plasma" remains unsolved. To enhance the feasibilities for noninvasive control of RBCs using laser radiation, we propose to excite an evanescent wave using a linearly polarized wave with an azimuth of polarization 45 deg, enabling realization of transversal spin flows in the evanescent wave and controlled spatial (transversal) motion of erythrocytes.
As a rule, various biological microobjects can be localized in different media. The control of such microobjects calls for the use of a large set of optical forces to provide directional action on the controlled object. These forces can be evaluated within the framework of the relevant optical theory. Theoretical models and experimental approaches approved up to now enable prognosing the behavior of objects of different shape, such as spheres, cylinders, and objects of more complex shapes, in optical traps and tweezers.
Here, we focus our attention on erythrocytes. The normal human erythrocyte has the shape of concavo-concave disc. The diameter of an erythrocyte is 7.82 AE 0.62 μm, and its minimal and maximal thickness at the center and at the limb are 0.81 AE 0.35 and 2.58 AE 0.27 μm, respectively. The mean magnitude of the cell volume of an erythrocyte is 94 AE 14 μm 3 , and the mean magnitude of the cell surface area is 135 AE 16 μm 2 . The mentioned sizes of an erythrocyte are estimated from measurings on photomicrographs. 18 The model of concavo-concave disc corresponds well to native erythrocytes experimentally proven. 19 Erythrocyte can be deformed in a shearing flow, where its shape becomes ellipsoidal. 20 Considering an erythrocyte as a uniforn disc with the mean erythrocyte volume, one can use the concept of single light-scattering by a cell where the cell organelles and the cell membrane are believed to be thin enough to neglect their influence of its real thckness on the light scattering properties. The Mie theory of light scattering applied for such cases provides a comprehensive description of the energy transfer to the erythrocyte. 18 • Modeling of an erythrocyte by a disc facilitates the determination of an algorithm that represents a cell in an evanescent. We are interested in understanding the transversal motion of such a cell. This becomes possible, if the probing wave is linearly polarized, with the azimuth of polarization AE45 deg. An elliptically polarized evanescent incident wave introduces a vertical spin angular momentum. This introduces transversal and longitudinal forces, inducing motion of the erythrocyte.
In contrast to previous studies of induced longitudinal motion induced by an evanescent field, we will here study transversal motion. This motion may be introduced by a combination of the incident field and its polarization. Therefore, rotational motion of an erythrocyte is not the subject of study in this work, while such motion has been investigated and experimentally proven. 21 Let us consider the theoretical model for exciting the transversal spin of an evanescent wave, as well as the densities of the spin and orbital flows compared with the experimentally observed rectilinear motion of a cell.
Observation of motion of isolated erythrocytes in whole blood is hampered by high concentration of cells. Concentration of erythrocytes is diminished using an isotonic solution. However, the simplest use of physiological solution is not practicable due to the lower viscosity of the solution changing the elasticity of erythrocytes. Consequently, we diminished the erythrocyte concentration using the natural medium of cells, viz. a blood plasma. Following this approach, both computer simulation and experimental demonstration of erythrocyte motion are carried out in blood plasma with refractive index (n sol ¼ 1.37). To realize the total internal reflection, we use a flint prism with an index of refraction n pr ¼ 1.61, above which an erythrocyte with the refraction index n er ¼ 1.407 is located 18 in the blood plasma. To avoid the temperature effects and irreversible temperature changes due to heating and destroying cells under illumination, one sets the power of radiation below 100 mW and the pulse cycling of about 1 μs.
An evanescent wave ( Fig. 1, III) formed above the prism surface ( Fig. 1, 1) penetrates into the erythrocyte (Fig. 1, 3) and creates an inhomogeneous intensity distribution in the XZplane, which is connected with the penetration depth for the The the angle of incidence of the beam at the prism surface is γ. By changing the angle of incidence and the wavelength, one can control this depth up to 0.9 μm. For the azimuth of polarization of the incident wave AE45 deg, the evanescent wave, being elliptically polarized, has a torque (τ) causing rotation of an erytrhocyte (Fig. 1).
The geometric parameters of an erythrocyte, 22,23 used for modeling the cell shape are: Estimations for objects of complex shape (such as an erythrocyte) through simple analytic computations of interaction of an evanescent wave and a cell are difficult, 23 being connected with considerable errors. Therefore, the following approach is proposed for modeling of an erythrocyte and determination of the conditions of interaction of a cell with an evanescent wave, here describing the erythrocyte shape as the set of facets of equal areas 0.01 μm 2 . The chosen size of the facets is necessary and sufficient for the description of the erythrocyte surface interacting with an evanecent wave. In respect to the transversal size h of an erythrocyte, 1∕3h takes part in the interaction of the cell and the evanescent wave. One computes the components of the optical force F x , F y , F z and the magnitude of torque τ for each facet. In doing so, one takes into account the angle of incidence of radiation at the corresponding facet and optical characteristics of the erythrocyte surface. Computer simulations were carried out for 3340 facets interacting with an evanescent wave adding up the derived components of the optical force for three directions for each facet. In the same way, one computes the resulting magnitude of torque. During simulation, the coordinates are obtained from the following equation describing the shape of an erythrocyte as a concavo-concave disc: 22 E Q -T A R G E T ; t e m p : i n t r a l i n k -; e 0 0 1 ; 3 2 6 ; 6 9 7 Correspondingly, the parameters P, Q, R, and S can be determined as E Q -T A R G E T ; t e m p : i n t r a l i n k -; s e c 2 ; 3 2 6 ; 6 3 4 Let us set the coordinates XYZ with the origin at point O (0,0,0), as it is shown in Fig. 2. The coordinates of facets are measured from this point to right and to left along the axes OY and OZ with the stepsize 0.01 μm. This step size provides the optimized condition for computer simulation. Following Eq. (1), for each ðy; zÞ position, one determines the magnitude x for the point at the erythrocyte surface. The areas of the facets chosen for simulation (∼0.01 μm 2 ) are determined using computer fitting. The upper magnitude of the coordinate along the X axis is chosen in correspondence with the preliminary estimated depth of penetration of an evanescent wave into the erythrocyte. The use of software Mathematica enables such division in triangular facets of equal areas, with determination of the apex of each facet, which is used for simulation of the erythrocyte surface. Using the mentioned software, one can estimate the coordinates of the each point of a facet, as well.
The obtained set of facets is distinct in its spatial position in the specified coordinates XYZ (Fig. 3). Each facet is marked by its normal orientation and by the angle of inclination of a facet, α i , to the YOZ plane. In addition, the facets are also different in the angle of rotation β i with respect to the XOZ plane.   Let us arbitrarily consider the i'th facet whose apexes are determined as A i ðx Ai ; y Ai ; z Ai Þ, B i ðx Bi ; y Bi ; z Bi Þ, and C i ðx Ci ; y Ci ; z Ci Þ. Then, the plane containing this facet can be determined as ; t e m p : i n t r a l i n k -; s e c 2 ; 6 3 ; 6 9 7 Here, the normal vector for the specified facet is determined byñ i ða i ; b i ; c i Þ. Then, the angle of inclination for the specified facet, α i , is given by , and the angle of p .
An evanescent wave propagating along the OZ direction reaches the specified i'th facet and makes some angle γ i with the normal to this facet at the point of incidence of a beam (Fig. 3). If the vector determining the direction of propagation of the incident beam is determined asbð0;0; c i Þ, then The three coordinates for the specified facet determine the point of incidence of a beam onto the facet, being arbitrarily chosen. Consequently, the normal and the incident beam determine the plane of incidence, and one can use the Fresnel equations to compute the components of the electrical field of the reflected and refracted waves, and to determine the angle of incidence, γ i , between the beam and the surface normal. Correspondingly, one can estimate the refraction angles, γ t i , for the incident beam at the each facet. The orientation of the plane of incidence with respect to the horizontal YOZ plane, i.e., the angle φ i , can be estimated by Here k z ¼ k n pr n sol sin γ is the propagation constant; is the exponential decay rate; m ¼ T ⊥ T ∕∕ m 1 is the state of polarization of an evanescent wave, 25 where m 1 is the state of polarization of the probing beam impinging on the interface "plate-air," being equal to unity for linear polarization with the azimuth of polarization 45 deg; γ is the incidence angle on the surface, where total internal refection takes place; and k is the wavenumber for the incident wave. The electrical field strength of an evanescent wave is is the transmission coefficient, 26 T ∕∕ and T ⊥ are the Fresnel transmission coefficients, and E 0 and ω are the strength and frequency of the electrical field of the incident wave, respectively. At the coordinates XYZ, for the plane of incidence one can write the components of the electrical vector of the reflected and refracted waves following the Fresnel equations. Therefore, for the reflected wave and the i'th facet one has E Q -T A R G E T ; t e m p : i n t r a l i n k -; s e c 2 ; 3 2 6 ; 6 3 0 Here,  Refs. 25 and 26, ½p s i ¼ 1 16πω ∇ × Im½½Ẽ tÃ ev i × ½Ẽ t ev i . Here, ½Ẽ t ev i is the transmitted evanescent wave interacting with the i'th facet of the erythrocyte. This expression contains both the longitudinal and transversal components. 22 The orbital angular momentum is given by ½p o i ¼ 1 8πω Im½½Ẽ tÃ ev i · ð∇Þ½Ẽ t ev i . The orbital (canonical) angular momentum caused by light pressure determines a force transferred to a cell in the direction of propagation of an evanescent wave.
Thus, the resulting momentum density in the OZ-direction is given as E Q -T A R G E T ; t e m p : i n t r a l i n k -; s e c 2 ; 6 3 ; 6 3 3 and the transversal momentum caused by the vertical spin Let us compute the optical forceF i acting on each facet of an erythrocyte. We use the Mie light-scattering theory, under which approximation, 26 where Δp i is the change of momentum density at the corresponding facet S i interacting with the evanescent wave for i ¼ 1 to N, where N is the number of facets. Thus, obtained Δ½p y i and Δ½p z i make it possible to calculate components of the optical force ½F Y i and ½F Z i .
The refracted evanescent wave in the OX-direction for each facet i also forms some component of the optical force in this direction, which can be computed using the relation: computed for the i'th facet in the OX-direction. Here, I is the unit matrix,n is the surface-normal vector, B t ev ¼ ffiffiffiffiffiffi ffi εε 0 p E t ev is the magnetic inductance vector for the evanescent wave, and ε is the media permittivity.
Inhomogeneous in intensity refracted evanescent wave caused by the action of the gradient force ½F grad i ¼ − 1 2 n∇½E t ev 2 i is connected with the direction of propagation of the beam refracted at the i'th facet of the beam. The total component of the optical force in the OX-direction for the i'th facet is ½F X i ¼ ½F x i þ ½F gradx i . The next calculation step consists in recalculation of the found set of optical force components for each of the specified facets, ½F X i , ½F Y i , ½F Z i , into the resulting components, by summation of the corresponding force components over all facets of the erythrocyte surface, The computed results show that the magnitude of the component of the force F X is not large enough to provide the vertical motion of an erythrocyte, which is by several orders of magnitude smaller than the magnitude of the longitudinal component, and therefore has no influence on the spatial motion of a cell.

Modeling of Controlled Transversal Motion of Erythrocyte
For observation of the controlled transversal motion of an erythrocyte in the YOZ-plane (which is possible only if the transversal components of the evanescent wave are active), we propose to implement the model arrangement shown in Fig. 5, where two oppositely directed evanescent waves of the same amplitude and frequency are formed above the prism. In the model experiment, the longitudinal components of the optical force of the evanescent waves onto the erythrocyte are compensated, so that only the transversal components are added. Let us emphasize, the discussed effect only becomes possible if one uses two linearly polarized incident waves with the azimuths of polarization AE45 deg with respect to the plane of incidence, so that two oppositely directed evanescent waves are of the same elliptical polarization.
In accordance with the above represented scheme for computation of the optical force generated by the evanescent wave, Fig. 4 Dependence of the magnitude of the torque resulting from action of an evanescent wave on an erythrocyte as a function of the angle of incidence of the laser beam onto the prism.
Journal of Biomedical Optics 055002-5 May 2019 • Vol. 24 (5) one determines the force components F m1 and F m2 for the first (I) and second (II) (Fig. 5) evanescent waves acting on the erythrocyte. As the preliminary simulation shows, the incidence of the beams onto the surface of a prism, where total internal reflection appears, must be symmetrical with respect to the axis of symmetry of an erythrocyte OO 0 . Let the polarizations of the two beams be the same, i.e., the azimuths of polarization of both beams are equal to þ45 deg or −45 deg. Thus, for the symmetrical incidence of the beams, both the total longitudinal component of the optical force, F Z ¼ F Z1 þ F Z2 , and the total transversal component, are the transversal and longitudinal components, respectively, of the evanescent waves excited by the linearly polarized incident waves with azimuths of polarization AE45 deg. Evanescent waves formed above the prism surface are elliptically polarized, with the same direction of rotation of the electric-field vector, each of which has torque, as has been shown above (cf. Figs. 1 and 4), which is inherent in the vertical spin of an evanescent wave. Such action results in the total torque that is twice as large for the chosen angles, see Fig. 4.
Formation of the transversal component of the optical force and demonstration of its directional action presumes the use of two linearly polarized incident beams whose azimuths of polarization are orthogonal, viz. þ45 deg and −45 deg (cf. Fig. 5). Elliptically polarized evanescent waves with the opposite directions of rotation of the electric field vectors compensate the resulting rotational motion of a cell and provide the condition for observation of a rectilinear motion of an erythrocyte.
In accordance with the conditions of modeling, for compensated longitudinal component F Z ¼ F Z1 þ F Z2 , one observes strengthening of the transversal component, which can be visualized experimentally. The magnitude of the transversal component and its influence on the characteristics of motion of a cell depend on the angle of incidence of waves on the erythrocyte surface and determine the velocity of a cell's spatial motion. In Fig. 6, we represent the graphic demonstration of changing transversal component of the force depending on the angle of incidence. Here, F Y ¼ F Y1 þ F Y2 . This enables variation of the spatial position of an erythrocyte. Let us assume that the transversal forces formed because of the action of an evanescent wave do not cause deformation of a moving erythrocyte. The maximal magnitude of the resulting optical force occurs for an angle of incidence of 63 deg. The parameters of erythrocyte shape used in simulation have been obtained from the experimental data. 20 In the previous, we have argued for the feasibility for control of spatial (transversal) motion of the studied microobject. Transversal motion of an erythrocyte in blood plasma is caused by the action of the resulting forceF ¼F opt þF st , where F opt ¼ F Y is the optical force. At low Reynolds numbers, the damping factor or drag coefficient can be estimated by approximating the disk shape of erythrocyte by a sphere of the same cross-sectional area, 29 then F st ¼ 3πηDv is the Stokes force. Here, η ¼ 1.236 × 10 −3 Pa · s is the dynamic viscosity of blood plasma, D is the diameter of the corresponding cross sectional area of the cell, and v is the velocity of an erytrhocyte. While m dv dt ¼ F þ 3πηDv, one can determine velocity of erythrocyte in a solution at the transversal direction as a function of time (Fig. 7), by comparing the theoretical and experimental results.
In the course of time, the velocity of an erythrocyte is experimentally detected while changing the angle of illumination.

Experimental Demonstration of Transversal Motion of Erythrocytes into Evanesent Field
The results of computer simulation of moving erythrocytes in an evanescent field will now be supported experimentally. A blood plasma 30 is prepared by centrifugation of fresh venous blood at room temperature. Initially, a whole blood (1500 g) is centrifuged during 10 min, which results in separation of sedimented   Here,k 1 ,k 2 are the wavevectors of the incident beams; F x 1 , F x2 , F y1 , F y 2 , F z1 , and F z2 are the components of the optical force of the evanescent waves E ev1 and E ev2 ; OO 0 is the axis of symmetry of an erythrocyte. N ¼ 1::i is the number of simulating facets.
Journal of Biomedical Optics 055002-6 May 2019 • Vol. 24 (5) erythrocytic masses from plasma. Further, a second centrifugation (1500 g) during 10 min is carried out at room temperature for removing residual cells, such as thrombocytes. Then, blood from a finger (0.5 μg) is diluted in 1 ml of blood plasma. The prepared suspension (50 μl) is placed in a hermetic cuvette consisting of a cover glass of 0.15 mm thickness and the total reflection prism at the distance 0.05 mm. To avoid adhesion of erythrocytes, a cover glass and the prism surface are sequentially washed out in a solution of surface-active material, in 96% ethyl alcohol, then in deionized water, and finally in 3% solution of hydrogen peroxide, and then dried and washed out in 2% solution of human albumin. The optical arrangement for the experimental demonstration of erythrocyte motion in an evanescent field is shown in Fig. 8.
The radiation from a semiconductor laser 1 with wavelength λ ¼ 980 nm and power 1 W passes the beam expander 2 consisting of two objectives and a micron-scale pinhole as the spatial frequency filter. We varied the radiation power from 5 to 500 mW. The beam with a plane wavefront passes a quarter-wave plate 3 and becomes circularly polarized. After beam splitter 4, two beams of equal intensities are reflected by the mirrors 5 to the total reflection prism 7. Polarizers 6 form the orthogonally linearly polarized beams with the azimuths of polarization 45 deg, which fall on the interface glass-blood plasma. In a cuvette 8 with erythrocytes, two oppositely directed evanescent waves of equal intensities are formed. Moving erythrocytes are observed in white light from a LED display 9 using a microobjective 10 (magnification 60 X ) and CCD-camera 12. Light filter 11 cuts off radiation from the IR laser.
We studied rectilinear motion of an erythrocyte in one evanescent wave, when the right beam in Fig. 8 is blocked (Fig. 9), and into two oppositely directed beams (Fig. 10). The motion of an erythrocyte in one evanescent beam is shown in Fig. 9 for the frame frequency 2 Hz and radiation power 100 mW. The speed of motion of an erythrocyte is about 35 μm∕s. An erythrocyte travels along the direction of propagation of the evanescent field. At the same time, one observes also some transversal motion of an erythrocyte with the speed 4 μm∕s, approximately, which is caused by the transversal component of the force F y influencing erythrocyte in the evanescent field. This situation was previously theoretically predicted.
Motion of an erythrocyte in opposite evanescent waves is shown in Fig. 10.
The frame frequency equals 1 Hz. One observes only transversal motion of an erythrocyte with a speed 6 μm∕s, approximately. The longitudinal moving is compensated by the opposite beams, while the transversal moving for linearly polarized beams with the azimuths of polarization AE45 deg doubles, in agreement with the theoretical prediction. One observes correlation of the results of computer simulation and the experimental study with a discrepancy not exceeding 30%.
Such a discrepancy is explained by the influence of various kinds of factors on the purity of the experiment. More specifically, the experimental solution with RBC was insufficiently clean, i.e., the presence of inhomogeneities giving rise to scattering was observed. This determined the second action from the evanescent wave on the object. In addition, our experimental realization assumed the change of the azimuth of polarization of the evanescent wave from AE45 deg (that was about 2% to 3%), which determined one of the possible causes of the experimental error as well.

Conclusion
A specially chosen theoretical model for exciting an evanescent wave and calculation of its impact on an erythrocyte has provided the conditions for rectilinear and rotational motion of  an isolated erythrocyte. It has been shown that, for the specified experimental conditions, the longitudinal motion of an erythrocyte is compensated, accompanied by an increasing transversal component of the optical force manifesting itself in controlled transversal red blood cell motion. The speed of transversal motion of an erythrocyte has been evaluated both theoretically and experimentally. Specifically, the discrepancy between the theoretical and experimental results does not exceed 30%.
Oleg V. Angelsky is a director of the Institute of Physical, Technical, and Computer Sciences, Chernivtsi National University, Ukraine. He is a fellow of the Institute of Physics (United Kingdom), SPIE, OSA, and EOS, and a member of an advisory committee for EOS. He is the author of about 300 publications, including some chapters of international books, for which he was an editor. His research interests are in rough surface characterization, fractal optics, holography, polarization optics, correlation optics, and singular optics.