Fiber orientation measurements by diffusion tensor imaging improve hydrogen-1 magnetic resonance spectroscopy of intramyocellular lipids in human leg muscles

Sunil K. Valaparla; Feng Gao; Giuseppe Daniele; Muhammad Abdul-Ghani; Geoffrey D. Clarke

doi:10.1117/1.JMI.2.2.026002

9 June 2015 Fiber orientation measurements by diffusion tensor imaging improve hydrogen-1 magnetic resonance spectroscopy of intramyocellular lipids in human leg muscles

Sunil K. Valaparla, Feng Gao, Giuseppe Daniele, Muhammad Abdul-Ghani, Geoffrey D. Clarke

Author Affiliations +

Journal of Medical Imaging, Vol. 2, Issue 2, 026002 (June 2015). https://doi.org/10.1117/1.JMI.2.2.026002

Abstract

Twelve healthy subjects underwent hydrogen-1 magnetic resonance spectroscopy (¹H-MRS) acquisition (15×15×15 mm³), diffusion tensor imaging (DTI) with a b-value of 600 s mm⁻², and fat-water magnetic resonance imaging (MRI) using the Dixon method. Subject-specific muscle fiber orientation, derived from DTI, was used to estimate the lipid proton spectral chemical shift. Pennation angles were measured as 23.78 deg in vastus lateralis (VL), 17.06 deg in soleus (SO), and 8.49 deg in tibialis anterior (TA) resulting in a chemical shift between extramyocellular lipids (EMCL) and intramyocellular lipids (IMCL) of 0.15, 0.17, and 0.19 ppm, respectively. IMCL concentrations were 8.66±1.24 mmol kg⁻¹, 6.12±0.77 mmol kg⁻¹, and 2.33±0.19 mmol kg⁻¹ in SO, VL, and TA, respectively. Significant differences were observed in IMCL and EMCL pairwise comparisons in SO, VL, and TA (p<0.05). Strong correlations were observed between total fat fractions from ¹H-MRS and Dixon MRI for VL (r=0.794), SO (r=0.655), and TA (r=0.897). Bland-Altman analysis between fat fractions (FF_MRS and FF_MRI) showed good agreement with small limits of agreement (LoA): bias=−0.21% (LoA: −1.12% to 0.69%) in VL, bias=0.025% (LoA: −1.28% to 1.33%) in SO, and bias=−0.13% (LoA: −0.74% to 0.47%) in TA. The results of this study demonstrate the variation in muscle fiber orientation and lipid concentrations in these three skeletal muscle types.

Citation Download Citation

Sunil K. Valaparla, Feng Gao, Giuseppe Daniele, Muhammad Abdul-Ghani, and Geoffrey D. Clarke "Fiber orientation measurements by diffusion tensor imaging improve hydrogen-1 magnetic resonance spectroscopy of intramyocellular lipids in human leg muscles," Journal of Medical Imaging 2(2), 026002 (9 June 2015). https://doi.org/10.1117/1.JMI.2.2.026002

Published: 9 June 2015

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available