One-step fabrication of three-dimensional polydimethylsiloxane peel-off microwell array for cell trapping

Ning Xue; Leonidas Bleris; Jeong-Bong Lee

doi:10.1117/1.JMM.14.1.014503

19 February 2015 One-step fabrication of three-dimensional polydimethylsiloxane peel-off microwell array for cell trapping

Ning Xue, Leonidas Bleris, Jeong-Bong Lee

Author Affiliations +

Journal of Micro/Nanolithography, MEMS, and MOEMS, Vol. 14, Issue 1, 014503 (February 2015). https://doi.org/10.1117/1.JMM.14.1.014503

Abstract

A three-dimensional polydimethylsiloxane (PDMS)-based microarray was one-step fabricated by releasing from an SU-8 mold for cell trapping. A human embryonic kidney-293 cell line was cultured on the PDMS microarray. By taking advantage of the chemical nature difference between PDMS surface released from oxidized silicon and released from SU-8 substrate, cells can only grow and be attached inside the PDMS microarray after 2 days of cell culture. To investigate the substantial nature of the cell adhesion and repellency on the PDMS substrate, atomic force microscopy, contact angle, and x-ray photoelectron spectroscopy methods have been carried out to analyze the physical and chemical properties of the PDMS material surfaces under different surface treatment circumstances.

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Ning Xue, Leonidas Bleris, and Jeong-Bong Lee "One-step fabrication of three-dimensional polydimethylsiloxane peel-off microwell array for cell trapping," Journal of Micro/Nanolithography, MEMS, and MOEMS 14(1), 014503 (19 February 2015). https://doi.org/10.1117/1.JMM.14.1.014503

Published: 19 February 2015

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